Identification of anti-inflammatory constituents from Kalimeris indica with UHPLC-ESI-Q-TOF-MS/MS and GC–MS. (13th May 2015)

• Record Type: Journal Article
• Authors: Zhong, Rui-Feng; Xu, Guo-Bo; Wang, Zheng; Wang, Ai-Min; Guan, Huan-Yu; Li, Jing; He, Xun; Liu, Jun-Hong; Zhou, Meng; Li, Yong-Jun; Wang, Yong-Lin; Liao, Shang-Gao
• Is Part Of: Journal of ethnopharmacology. Volume 165(2015)
• Page Start: 39
• Page End: 45
• Publication Date: 2015-05-13
• Subjects: 3, 4-dicaffeoylquinic acid (PubChem CID: 5281780) -- 3, 5-dicaffeoylquinic acid (PubChem CID: 6474310) -- 1, 5-dicaffeoylquinic acid (PubChem CID 29805) -- Rutin (PubChem CID: 5280805) -- 1-malonyl-3, 5-dicaffeoylquinic acid (PubChem CID: 6474367) -- 4, 5-dicaffeoylquinic acid (6), 13-tetradecenal (PubChem CID: 522841) -- (Z, Z)-9, 12-octadecadienoic acid (PubChem CID: 5280450) -- (3α)-12-oleanen-3-yl acetate (9), (+)-3-oxo-urs-12-en-24-oic acid methyl ester (10); Kalimeris indica -- Asteraceae -- Active fractions -- Dicaffeoylquinic acids -- Anti-inflammatory activity; Ethnopharmacology -- Periodicals; Pharmacognosy -- Periodicals; Herbs -- Periodicals; Herbs -- Periodicals; Pharmacognosy -- Periodicals; Pharmacognosie -- Périodiques; Herbes -- Périodiques; 615.1
• DOI: 10.1016/j.jep.2015.02.034
• Languages: English
• ISSNs: 0378-8741

Abstract

Abstract: Ethnopharmacological relevance: Kalimeris indica is a Miao׳s medicinal plant in Guizhou province of China employing to treat various inflammation-related diseases in clinical. The study aims to determine the active fractions of K. indica for its anti-inflammatory activity and to identify their chemical constituents. Material and methods: The dried K. indica herb was extracted with 50% aqueous ethanol and then successively separated with macroporous resin and MCI column chromatography to give five fractions (A–E). The anti-inflammatory effects were determined by measuring the NO and TNF-α production in murine macrophage RAW 264.7 cells after exposure to LPS. The chemical constituents of the anti-inflammatory fractions were analyzed by the method of UHPLC-ESI-Q-TOF/MS or GC–MS. Results: Five fractions (A–E) of different polarities were prepared from the 50% ethanol extract. Factions C and E showed significant inhibition of NO and TNF-α production. Six constituents, namely 3, 4-dicaffeoylquinic acid (1 ), 3, 5-dicaffeoylquinic acid (2 ), 1, 5-dicaffeoylquinic acid (3 ), rutin (4 ), 1-malonyl-3, 5-dicaffeoylquinic acid (5 ), and 4, 5-dicaffeoylquinic acid (6 ) were identified from the active fraction C by UHPLC-ESI-Q-TOF/MS. Four compounds including 13-tetradecenal (7 ), ( Z, Z )-9, 12-octadecadienoic acid (8 ), (3α)-12-oleanen-3-yl acetate (9 ), and (+)-3-oxo-urs-12-en-24-oic acid methyl ester (10 ) were identified from the active fraction E by GC–MS. Conclusion: K. indica possessed pronounced anti-inflammatory effect. Dicaffeoylquinic acids and their dirivatives, rutin, as well as oleanolic and fatty acid derivatives are the major constituents and possibly the anti-inflammatory principles of the active fractions of K. indica . All the compounds were identified in K. indica for the first time. The work provided evidence for further development and utilization of K. indica and formed a basis for the establishment of quality control methods and standards for K. indica and its pharmaceutical preparations. Graphical abstract: