Back Cover: Stepwise "bridge‐to‐bridge" reduction of monoclonal antibodies and light chain detection: Case studies of tenatumomab and trastuzumab. Issue 4 (17th April 2018)

• Record Type: Journal Article
• Authors: Mazzoccanti, Giulia; Pierri, Giuseppe; Ciogli, Alessia; Ismail, Omar H.; Giorgi, Fabrizio; De Santis, Rita; Villani, Claudio; Gasparrini, Francesco
• Is Part Of: Separation science plus. Volume 1:Issue 4(2018)
• Page Start: NA
• Page End: NA
• Publication Date: 2018-04-17
• Subjects: Chromatographic analysis -- Periodicals; 543.8
• DOI: 10.1002/sscp.201870015
• Languages: English
• ISSNs: 2573-1815

Abstract

Abstract : Sep Sci plus 2018, 1, 261–269 DOI:10.1002/sscp.201800002 The cover picture shows the chromatographic separation of reduced light chain isoforms upon incubation of antibodies with the reducing agent tris (2‐carboxyethyl) phosphine. Light chain, partially reduced light chain+2H (with one of two disulfide bridges opened in the constant or variable region) and totally reduced light chain+4H can be observed. The method was applied in the case of two monoclonal antibodies, namely tenatumomab and trastuzumab, resulting in a potential tool to detect disulfide bridges variants.