Temperature and pH Controlled Self‐Assembly of a Protein–Polymer Biohybrid. Issue 7 (22nd January 2018)

• Record Type: Journal Article
• Authors: Jiang, Lingdong; Bonde, Johan Svensson; Ye, Lei
• Is Part Of: Macromolecular chemistry and physics. Volume 219:Issue 7(2018)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2018-01-22
• Subjects: amelogenin -- atom transfer radical polymerization -- bioconjugate -- dual‐responsive -- self‐assembly; Polymers -- Periodicals; Polymerization -- Periodicals; Synthetic products -- Periodicals; Macromolecules -- Periodicals; 547.7
• DOI: 10.1002/macp.201700597
• Languages: English
• ISSNs: 1022-1352

Abstract

Abstract: A novel pH and temperature dual‐responsive bioconjugate is prepared by grafting thermoresponsive polymer chains from a pH‐responsive protein amelogenin via atom transfer radical polymerization. To the best of our knowledge, this is the first time that amelogenin is exploited to prepare a hybrid biomaterial with new stimuli‐responsive property. In both basic and acidic solutions the protein–polymer bioconjugate is able to self‐assemble into uniform and stable nanoparticles when heated to above the lower critical solution temperature of the polymer. The amelogenin‐based stimuli‐responsive bioconjugate may be of great use in the fields of bioseparation and drug/gene delivery, and the synthetic approach reported here should provide a convenient means to preparing amelogenin‐based functional biohybrid materials. Abstract : A novel pH and temperature dual‐responsive protein–polymer conjugate is prepared by grafting poly( N ‐isopropylacrylamide) chains from the pH‐responsive protein amelogenin via atom transfer radical polymerization technique. This hybrid biopolymer is able to self‐assemble into highly uniform nanoparticles by adjusting environmental conditions and is promising for applications in bioseparation and drug delivery fields.