Mouse GTSF1 is an essential factor for secondary piRNA biogenesis. (7th February 2018)

• Record Type: Journal Article
• Authors: Yoshimura, Takuji; Watanabe, Toshiaki; Kuramochi‐Miyagawa, Satomi; Takemoto, Noriaki; Shiromoto, Yusuke; Kudo, Akihiko; Kanai‐Azuma, Masami; Tashiro, Fumi; Miyazaki, Satsuki; Katanaya, Ami; Chuma, Shinichiro; Miyazaki, Jun‐ichi
• Is Part Of: EMBO reports. Volume 19:Number 4(2018)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2018-02-07
• Subjects: ping‐pong cycle -- piRNAs -- PIWI -- secondary piRNA biogenesis -- transposon silencing; Molecular biology -- Periodicals; Molecular Biology -- Periodicals; Molecular biology; Periodicals; 572.8
• DOI: 10.15252/embr.201642054
• Languages: English
• ISSNs: 1469-221X

Abstract

Abstract: The piRNA pathway is a piRNA‐guided retrotransposon silencing system which includes processing of retrotransposon transcripts by PIWI‐piRNAs in secondary piRNA biogenesis. Although several proteins participate in the piRNA pathway, the ones crucial for the cleavage of target RNAs by PIWI‐piRNAs have not been identified. Here, we show that GTSF1, an essential factor for retrotransposon silencing in male germ cells in mice, associates with both MILI and MIWI2, mouse PIWI proteins that function in prospermatogonia. GTSF1 deficiency leads to a severe defect in the production of secondary piRNAs, which are generated from target RNAs of PIWI‐piRNAs. Furthermore, in Gtsf1 mutants, a known target RNA of PIWI‐piRNAs is left unsliced at the cleavage site, and the generation of secondary piRNAs from this transcript is defective. Our findings indicate that GTSF1 is a crucial factor for the slicing of target RNAs by PIWI‐piRNAs and thus affects secondary piRNA biogenesis in prospermatogonia. Synopsis: Mouse GTSF1 is essential for MILI‐directed secondary piRNA biogenesis in prospermatogonia and associates with the MIWI2 complex probably to transcriptionally silence its targets. Mouse GTSF1 associates with MILI, MIWI, and MIWI2 complexes. Mouse GTSF1 deficiency leads to loss of MIWI2‐bound piRNAs and to a severe defect in secondary piRNA biogenesis, whereas it has no impact on primary piRNA biogenesis. In Gtsf1 mutants, a known target RNA of PIWI‐piRNAs is left unsliced at the cleavage site, and the generation of secondary piRNAs from this transcript is defective. Abstract : Mouse GTSF1 is essential for MILI‐directed secondary piRNA biogenesis in prospermatogonia and associates with the MIWI2 complex probably to transcriptionally silence its targets.