Cordyceps cicadae NTTU 868 mycelium prevents CCl4-induced hepatic fibrosis in BALB/c mice via inhibiting the expression of pro-inflammatory and pro-fibrotic cytokines. (April 2018)

• Record Type: Journal Article
• Authors: Ke, Bo-Jun; Lee, Chun-Lin
• Is Part Of: Journal of functional foods. Volume 43(2018)
• Page Start: 214
• Page End: 223
• Publication Date: 2018-04
• Subjects: Hepatic fibrosis -- Cordyceps cicadaeNTTU 868 -- Carbon tetrachloride -- Adenosine -- N6-(2-hydroxyethyl)-adenosine (HEA) and polysaccharides; ALT alanine aminotransferase -- AST aspartate aminotransferase -- BUN blood urea nitrogen -- CAT catalase -- CCl4 carbon tetrachloride -- CTGF connective tissue growth factor -- DMN dimethylnitrosamine -- ECM extracellular matrix -- GPx glutathione peroxidase -- GRd glutathione reductase -- H&E stain hematoxylin and eosin stain -- HEA N6-(2-hydroxyethyl)-adenosine -- HGF hepatocyte growth factor -- HSCs hepatic satellites cells -- HYP hydroxyproline -- ICAM-1 intercellular adhesion molecule 1 -- IL-1β interleukin-1β -- IL-6 interleukin-6 -- IP injection intraperitoneal injection -- IPS intracellular polysaccharides -- MAPKs mitogen-activated protein kinases -- MMPs matrix metalloproteinases -- NF-κB Nuclear factor-κB -- PIIIP procollagen-III peptide -- PPAR-γ peroxisome proliferator-activated receptor gamma -- PSR stain picro-sirius red stain -- ROS reactive oxygen species -- SCr serum creatinine -- SOD superoxide dismutase -- TAA thioacetamide -- TBA thiobarbituric acid -- TBARS thiobarbituric acid reactive substances -- TGF-β1 transforming growth factor-β1 -- TIMPs tissue inhibitor of metalloproteinases -- TNF-α tumor necrosis factor-α -- α-SMA α-smooth muscle actin; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2018.02.010
• Languages: English
• ISSNs: 1756-4646

Abstract

Graphical abstract: Highlights: C. cicadae NTTU 868 mycelium prevented CCl4 -induced liver fibrosis. Adenosine, HEA and polysaccharides prevented CCl4 -induced liver fibrosis. C. cicadae prevented liver fibrosis via TNF-α/IL-6 pathway, TGF-β1/CTGF pathway. Abstract: Hepatic fibrosis is caused by chronic liver injury and repeated repair with scar tissue accumulation in the liver. Cordyceps cicadae produces large quantities of adenosine, N6-(2-hydroxyethyl)-adenosine (HEA) and polysaccharides. This study investigated the effect of C. cicadae NTTU 868 mycelium and its bioactive compounds on carbon tetrachloride (CCl4 )-induced liver fibrosis. BALB/c mice were injected 0.5 µL/g CCl4 3 times weekly for 4 weeks to induce hepatic fibrosis and treated with C. cicadae NTTU 868 mycelium, adenosine, HEA, and polysaccharides daily for 6 weeks. CCl4 administration not only caused a remarkable liver damage but also resulted in abnormal collagen accumulation. C. cicadae NTTU 868 mycelium prevented CCl4 -induced hepatic fibrosis through TNF-α/IL-6 pathway, TGF-β1/CTGF pathway, and anti-oxidative defense mechanism. Although adenosine, HEA and polysaccharides performed protection against CCl4 -induced liver damage, HEA should be the main bioactive compound on the prevention of hepatic fibrosis in the C. cicadae NTTU 868 mycelium.