Chemical characterization and bioactive properties of decoctions and hydroethanolic extracts of Thymus carnosus Boiss. (April 2018)

• Record Type: Journal Article
• Authors: Martins-Gomes, Carlos; Taghouti, Meriem; Schäfer, Judith; Bunzel, Mirko; Silva, Amélia M.; Nunes, Fernando M.
• Is Part Of: Journal of functional foods. Volume 43(2018)
• Page Start: 154
• Page End: 164
• Publication Date: 2018-04
• Subjects: ABTS 2, 2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) -- CAE Caffeic acid equivalents -- DAD Diode array detector -- DMEM Dulbecco's Modified Eagle Medium -- EDTA Ethylenediaminetetraacetic acid -- ER Oestrogen receptor -- ESI electrospray ionization -- FBS Fetal bovine serum -- GAE Gallic acid equivalents -- H1D first harvest of decoction extracts -- H1HE first harvest of hydroethanolic extracts -- H2D second harvest of decoction extracts -- H2HE second harvest of hydroethanolic extracts -- HER2 receptor tyrosine-protein kinase -- LC-MS liquid chromatography coupled to mass spectrometry -- LPS Lipopolysaccharide -- PI3K Phosphatidylinositol 3-kinase -- RP-HPLC reversed-phase high performance liquid chromatography -- TBA Thiobarbituric acid; Caffeic acid (PubChem CID: 689, 043) -- Eriodictyol (PubChem CID: 440, 735) -- Luteolin (PubChem CID: 5, 280, 445) -- Rosmarinic acid (PubChem CID: 5, 281, 792) -- Salvianic acid A (PubChem CID: 11, 600, 642) -- Salvianolic acid A (PubChem CID: 5, 281, 793) -- Ursolic acid (PubChem CID: 64, 945) -- Oleanolic acid (PubChem CID:10, 494); Thymus carnosus Boiss. -- Phenolic compounds profile -- Antioxidant activity -- Anti-inflammatory activity -- Bioactivity -- Cytotoxicity; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2018.02.012
• Languages: English
• ISSNs: 1756-4646

Abstract

Graphical abstract: Highlights: Thymus carnosus Boiss. phytochemical profile was studied for the first time. A salvianolic acid A isomer was described for the first time in Thymus genus. Salvianolic A isomer and K, ursolic and oleanolic acids were the main components. Decoction and hydroethanolic extracts were cytotoxic to MCF-7 and BT-474 cell lines. Extracts possess antioxidant, anti-inflammatory and anti-proliferative activities. Abstract: Thymus carnosus Boiss. is an endangered species endemic to Portugal. Studies on its phytochemical composition and bioactivity would increase its use and protection, as no data is available. LC-MS analysis of T. carnosus decoction and hydroethanolic extracts allowed the identification of a salvianolic A isomer, which has not been described in Thymus genus before, rosmarinic and salvianolic K acids as the main phenolics. Luteolin, apigenin and eriodictyol glycosides were identified in lower amounts. Oleanolic and ursolic acids were present only in hydroethanolic extracts. Decoction extracted 32.4% of the phenolic compounds obtained by exhaustive hydroethanolic extraction. Both extracts reduced the viability of three cancer cell lines (human breast: MCF-7, BT-474; murine macrophage: Raw 264.7), with hydroethanolic extracts showing a higher activity. T. carnosus extracts possess significant antioxidant, anti-inflammatory and anti-proliferative activities and may be suggested as a new potential source of natural antioxidants and their decoctions as a novel functional food.