Cytotoxic, genetic and statistical analytical evaluation of functionalized CNTs with C2C12 cells. (June 2018) - Wahab, Rizwan
Abstract: The present work was designed to apprehend the cytotoxic and genetic evaluation study of multiwalled carbon nanotubes (MWCNTs). The CNTs were surface functionalized via HNO3 and H2 SO4 digestion process, confirmed via FTIR spectroscopy whereas the structure of CNTs was visualized with TEM. The obtained CNTs structures were utilized to control the proliferation rate of C2C12 cells. The cells were cultivated and % viabile and non viable cells % were checked by MTT assay with the influence of dose-dependent (500 ng/mL, 1000 ng/mL, 100 μg/mL) CNTs. Further the density of live and dead cells in liquid was also clearifies via confocal laser scanning microscopy (CLSM). Containing these, the genetic study to know the apoptosis caused due to CNTs were checked in presence of caspases 3 and 7 genes. The up-regulation data clearly favours the CNTs are responsible in cells death in C2C12 with the treatment at low (500, ng/mL) and (1000 ng/mL) high dosese. The statistical studies were also performed to authenticate the data for CNTs with different optimized parameters such as detection limit at low concentrations level, recoveries and relative standard deviation at measures 95% confidence level. The analyses palyses a significant role on C2C12 cells with the large effect of CNTs. Highlights: The twisted ropes of CNTs were surface functionalized via acid treatment method. Cytotoxic and genetic evaluations were studies with myoblast cells. Cells death was studies with MTT and confocal microscopy. The RT-PCR results show the up-regulation in mRNA level in caspase 3/7 with the influence of CNTs. The data also validated with statistical analytical studies.