Combined effect of retinoic acid and calcium on the in vitro differentiation of human adipose-derived stem cells to adipocytes. (15th March 2018)

• Record Type: Journal Article
• Authors: Goudarzi, Farjam; Sarveazad, Arash; Mahmoudi, Maryam; Mohammadalipour, Adel; Chahardoli, Reza; Malekshah, Obeid M.; Karimi Gorgani, Shiva; Saboor-Yaraghi, Ali Akbar
• Is Part Of: Archives of physiology and biochemistry. Volume 124:Number 2(2018)
• Page Start: 109
• Page End: 118
• Publication Date: 2018-03-15
• Subjects: ATRA -- adipogenesis -- hADSCs -- PPARG2 -- GLUT4; Physiology -- Periodicals; Biochemistry -- Periodicals; Biophysics -- Periodicals; Biochemistry; Physiology; 571
• DOI: 10.1080/13813455.2017.1367009
• Languages: English
• ISSNs: 1381-3455

Abstract

Abstract: Context: It has been shown that adipogenesis can be modulated by factors such as all-trans retinoic acid (ATRA) and calcium. Objective: To determine, the combined effect of ATRA and calcium on the differentiation of human adipose-derived stem cells (hADSCs). Methods: Mesenchymal stem cells (MSCs) were differentiated into the adipocytes by 0.5 and 1 µM of ATRA and 5 and 10 mM calcium separately or in combination. After MTS assay the differentiation of MSCs to adipocyte was evaluated, Oil Red O staining, GLUT4 concentration and gene expression of PPARG2, adiponectin, and GLUT4 were measured by Real-Time PCR. Results: Except 10 mM calcium treated group, other groups and more significantly combination treatments could reduce all adipocyte markers compared to the control. Conclusion: These results suggest that ATRA and calcium together have significant inhibitory effect on adipogenesis that can be helpful for finding new mechanisms to prevent or control the adipogenesis.