Cryogenic IR spectroscopy combined with ion mobility spectrometry for the analysis of human milk oligosaccharides. Issue 8 (15th March 2018)

• Record Type: Journal Article
• Authors: Khanal, Neelam; Masellis, Chiara; Kamrath, Michael Z.; Clemmer, David E.; Rizzo, Thomas R.
• Is Part Of: Analyst. Volume 143:Issue 8(2018)
• Page Start: 1846
• Page End: 1852
• Publication Date: 2018-03-15
• Subjects: Chemistry, Analytic -- Periodicals; 543
• DOI: 10.1039/c8an00230d
• Languages: English
• ISSNs: 0003-2654

Abstract

Abstract : Cryogenic, messenger-tagging, infrared spectroscopy is combined with ion mobility spectrometry and mass spectrometry to identify isomeric human milk oligosaccharides (HMOs) ranging from trisaccharides to hexasaccharides. Abstract : We report here our combination of cryogenic, messenger-tagging, infrared (IR) spectroscopy with ion mobility spectrometry (IMS) and mass spectrometry (MS) as a way to identify and analyze a set of human milk oligosaccharides (HMOs) ranging from trisaccharides to hexasaccharides. The added dimension of IR spectroscopy provides a diagnostic fingerprint in the OH and NH stretching region, which is crucial to identify these oligosaccharides, which are difficult to distinguish by IMS alone. These results extend our previous work in demonstrating the generality of this combined approach for distinguishing subtly different structural and regioisomers of glycans of biologically relevant size.