Application of electrophoretic deposition to occlude dentinal tubules in vitro. (April 2018)

• Record Type: Journal Article
• Authors: Zhang, Lu; Sun, Hualing; Yu, Jian; Yang, Hongye; Song, Fangfang; Huang, Cui
• Is Part Of: Journal of dentistry. Volume 71(2018:Apr.)
• Page Start: 43
• Page End: 48
• Publication Date: 2018-04
• Subjects: Dentin hypersensitivity -- Electrophoretic deposition -- Mesoporous silica -- Dentin permeability -- Dentinal tubule -- Shear bond strength; Dentistry -- Periodicals; Dentistry -- Periodicals; Dentisterie -- Périodiques; Electronic journals; 617.6005
• DOI: 10.1016/j.jdent.2018.01.012
• Languages: English
• ISSNs: 0300-5712

Abstract

Abstract: Objectives: This study aims to apply electrophoretic deposition (EPD) for occlusion of dentinal tubules in vitro and investigate its effect on tubule occlusion and shear bond strength (SBS). Methods: Charged mesoporous silica nanoparticles (MSNs) were synthesized and characterized through field-emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM), dynamic light scattering (DLS), and Fourier transform infrared (FT-IR) spectroscopy analyses. Thirty-nine sensitive dentin specimens were modeled and assigned randomly to three groups with different treatments ( n = 13 each): group 1, immersion in the MSN suspension; and groups 2 and 3, anodic EPD with the specimen on the negative and positive electrode respectively. The effect of dentinal tubule occlusion was evaluated by dentin permeability test ( n = 10 each) and FESEM examination ( n = 3 each). Moreover, 18 specimens were grouped ( n = 6 each) and treated in the same method. A resin stick was bonded onto each of the specimen using a self-etch adhesive (single bond universal) for SBS testing. Results: Negatively-charged MSNs were synthesized and characterized as small and well-dispersed particles. After the EPD treatment (group 3), the dentinal tubules were effectively occluded by MSNs, which infiltrated into the tubules at a depth of approximately 7–8 μm and tightly associated with the tubular inwalls. SBS was not significantly different among the three groups ( P > 0.05). Conclusions: Synthesized MSNs were deposited into dentinal tubules by EPD treatment without compromising dentin bond strength. Clinical significance: Application of EPD is a new approach for occlusion of dentinal tubules and exhibits potential in the study of dentin hypersensitivity.