Effects of PEG-induced drought stress on regulation of indole alkaloid biosynthesis in Catharanthus roseus. Issue 1 (1st January 2017)

• Record Type: Journal Article
• Authors: Liu, Ying; Meng, Qinghuan; Duan, Xihua; Zhang, Zhonghua; Li, Dewen
• Is Part Of: Journal of plant interactions. Volume 12:Issue 1(2017)
• Page Start: 87
• Page End: 91
• Publication Date: 2017-01-01
• Subjects: Catharanthus roseus -- alkaloids -- PEG -- vindoline -- catharanthine -- vinblastine; Plant ecophysiology -- Periodicals; Plants -- Periodicals; Toxins -- Periodicals; 581.7
• DOI: 10.1080/17429145.2017.1293852
• Languages: English
• ISSNs: 1742-9145

Abstract

ABSTRACT: Catharanthus roseus ( C. roseus ) plants were used to investigate the terpenoid indole alkaloids (TIAs) accumulation under the condition of PEG-induced drought stress. Multivariate analysis showed that 35% PEG6000 could induce more obvious and stable accumulation on proline (PRO) content and the relative water content (RWC). The results indicated that there were gradual increase and then decrease ( p < .05) in the contents of vindoline (VIN) and catharanthine (CAT) under 35% PEG6000 stress, but the content of vinblastine (VBL) increased gradually. In addition, the expression levels of tryptophan decarboxylase (TDC), strictosidine synthase (STR) and deacetylvindoline-4-O-acetyltransferase (DAT) were upregulated in plants under 35% PEG6000 stress. Further correlation analysis indicated that CAT accumulation was significantly correlated with TDC gene expression, and VBL accumulation was significantly correlated with peroxidase ( p < .05). Our results suggest that the cultivation of C. roseus in drought stress would serve as effective treatment for accumulating TIAs.