Temporospatial expression of fibulin-1 after acute spinal cord injury in rats. (November 2015)

• Record Type: Journal Article
• Authors: Xu, Guanhua; Cui, Ying; Wang, Lingling; Zhang, Jinlong; Shen, Aiguo; Li, Weidong; Bao, Guofeng; Sun, Yuyu; Cui, Zhiming
• Is Part Of: Journal of spinal cord medicine. Volume 38:Number 6(2015:Nov.)
• Page Start: 709
• Page End: 716
• Publication Date: 2015-11
• Subjects: Fibulin-1 -- Glial proliferation -- Neuron apoptosis -- Rat -- Spinal cord injury; Spinal cord -- Wounds and injuries -- Periodicals; Spinal cord -- Diseases -- Periodicals; 616.8305
• DOI: 10.1179/2045772314Y.0000000228
• Languages: English
• ISSNs: 1079-0268

Abstract

Abstract : Objective: Fibulin-1 is a matricellular protein that plays important roles in motility inhibition in a variety of cells and blocks the proliferation of cultured neural stem cells. The biological function of fibulin-1 in the spinal cord has not been fully elucidated. Methods: To clarify the expressions and possible functions of fibulin-1 in spinal cord injury (SCI), we performed an acute spinal cord contusion injury model in adult rats. Our work studied the temporospatial expression patterns of fibulin-1. Results: Western blot analysis revealed that fibulin-1 levels significantly increased 5 days after spinal cord contusion. Immunohistochemistry confirmed an increased number of fibulin-1 immunopositive cells about 2 mm from the lesion site. Moreover, double immunofluorescence labeling suggested that these changes were especially prominent in neurons and microglia. Conclusion: These findings suggest that fibulin-1 may be involved in neuronal apoptosis and microglial activation after SCI.