The inhibition of Pseudomonas aeruginosa biofilm formation by micafungin and the enhancement of antimicrobial agent effectiveness in BALB/c mice. Issue 7 (8th August 2016)

• Record Type: Journal Article
• Authors: Kissoyan, Kohar Annie B.; Bazzi, Wael; Hadi, Usamah; Matar, Ghassan M.
• Is Part Of: Biofouling. Volume 32:Issue 7(2016)
• Page Start: 779
• Page End: 786
• Publication Date: 2016-08-08
• Subjects: Micafungin -- biofilm -- Pseudomonas aeruginosa -- antibacterial agents -- BALB/c mice; Marine fouling organisms -- Periodicals; Materials -- Biodeterioration -- Periodicals; Fouling -- Periodicals; Biodegradation -- Periodicals; Environmental Microbiology -- Periodicals; Water Microbiology -- Periodicals; 576.165
• DOI: 10.1080/08927014.2016.1199021
• Languages: English
• ISSNs: 0892-7014

Abstract

Abstract: Micafungin inhibits biofilm formation by impeding 1, 3-β-D-glucan synthesis in Candida albicans . Since Pseudomonas aeruginosa also has 1, 3-β-D-glucan in its cell wall, this study assessed the effects of antibacterial agents in vitro and in vivo on micafungin-treated biofilm-forming P. aeruginosa isolates. After treatment with micafungin as well as with a panel of four antibacterial agents, biofilm production was significantly reduced as measured by spectrophotometry. The relative mRNA transcription levels for the genes encoding pellicles ( pelC ) and cell wall 1, 3-β-D-glucan ( ndvB ), which were measured by quantitative reverse transcription PCR (qRT-PCR), significantly decreased with micafungin treatment. In vivo, the survival rates of P. aeruginosa -infected BALB/c mice significantly increased after combined treatment with micafungin and each of the antibacterial agents. Of these treatments, the combination of micafungin with levofloxacin had the highest survival rate; this combination was the most effective treatment against P. aeruginosa -induced infection.