The degradation of three-ringed polycyclic aromatic hydrocarbons by wood-inhabiting fungus Pleurotus ostreatus and soil-inhabiting fungus Agaricus bisporus. Issue 5 (May 2018)

• Record Type: Journal Article
• Authors: Pozdnyakova, Natalia; Dubrovskaya, Ekaterina; Chernyshova, Marina; Makarov, Oleg; Golubev, Sergey; Balandina, Svetlana; Turkovskaya, Olga
• Is Part Of: Fungal biology. Volume 122:Issue 5(2018)
• Page Start: 363
• Page End: 372
• Publication Date: 2018-05
• Subjects: Agaricus bisporus -- Biodegradation -- Laccase -- Pleurotus ostreatus -- Polycyclic aromatic hydrocarbons (PAHs) -- Versatile peroxidase; Mycology -- Periodicals; Fungi -- Periodicals; 579.505
• DOI: 10.1016/j.funbio.2018.02.007
• Languages: English
• ISSNs: 1878-6146

Abstract

Abstract: The degradation of two isomeric three-ringed polycyclic aromatic hydrocarbons by the white rot fungus Pleurotus ostreatus D1 and the litter-decomposing fungus Agaricus bisporus F-8 was studied. Despite some differences, the degradation of phenanthrene and anthracene followed the same scheme, forming quinone metabolites at the first stage. The further fate of these metabolites was determined by the composition of the ligninolytic enzyme complexes of the fungi. The quinone metabolites of phenanthrene and anthracene produced in the presence of only laccase were observed to accumulate, whereas those formed in presence of laccase and versatile peroxidase were metabolized further to form products that were further included in basal metabolism (e.g. phthalic acid). Laccase can catalyze the initial attack on the PAH molecule, which leads to the formation of quinones, and that peroxidase ensures their further oxidation, which eventually leads to PAH mineralization. A . bisporus, which produced only laccase, metabolized phenanthrene and anthracene to give the corresponding quinones as the dominant metabolites. No products of further utilization of these compounds were detected. Thus, the fungi's affiliation with different ecophysiological groups and their cultivation conditions affect the composition and dynamics of production of the ligninolytic enzyme complex and the completeness of PAH utilization. Graphical abstract: Highlights: Degradation of phenanthrene and anthracene by wood- and soil-inhabiting basidiomycetes followed the same scheme. The fate of quinone metabolites is determined by the composition of the ligninolytic enzyme complexes of the fungi. Laccase can catalyze the initial attack on the PAH molecule, leading to the formation of quinones. Peroxidase ensures the further oxidation of the formed quinones leading to their involvement to basal metabolism.