Cytotoxic and inflammatory effects of alendronate and zolendronate on human osteoblasts, gingival fibroblasts and osteosarcoma cells. Issue 4 (April 2018) - Açil, Yahya
Abstract: Objective: The aim of this paper was to assess the effects of zoledronate (ZOL) and alendronate (FOS) on apoptotic behavior and gene expression of pro- and inflammatory cytokines of three cell types (human osteoblasts, human gingival fibroblasts and human osteogenic sarcoma cell lines) during a period of 4 weeks. Material and methods: Cell viability and proliferation was assessed via cell proliferation test (MTT), fluorescence diacetate analysis (FDA). Expression of inflammatory cytokines was investigated by using polymerase chain reaction. Results: The proliferation and cell vitality of osteoblasts and fibroblasts were negatively affected in a dose dependent manner under ZOL and FOS administration. Osteosarcoma cells showed an increase in proliferation under lower doses of BP. ZOL had a significantly higher cytotoxic effect compared with FOS on osteoblasts and fibroblasts. ZOL increased the production of IL-6 in all cell types, whereas FOS only in osteosarcoma cell, which happened in dose dependent manner. Bisphosphonates could result in increase of IL-1β expression of fibroblasts. An increase of IL-12 was observed at higher doses of ZOL administration among osteoblasts and FOS administration in osteosarcoma cells. Conclusion: ZOL and FOS could encourage cytotoxic and inflammatory reactions.