Diversity of antimicrobial resistance and virulence genes in methicillin-resistant non-Staphylococcus aureus staphylococci from veal calves. (April 2015)

• Record Type: Journal Article
• Authors: Argudín, M. Angeles; Vanderhaeghen, Wannes; Butaye, Patrick
• Is Part Of: Research in veterinary science. Volume 99(2015)
• Page Start: 10
• Page End: 16
• Publication Date: 2015-04
• Subjects: Coagulase negative staphylococci -- Epidemiology -- Zoonotic; Veterinary medicine -- Periodicals; Veterinary Medicine -- Periodicals; Médecine vétérinaire -- Périodiques; Médecine vétérinaire -- Recherche -- Périodiques; Diergeneeskunde; 636.089
• DOI: 10.1016/j.rvsc.2015.01.004
• Languages: English
• ISSNs: 0034-5288

Abstract

Highlights: Methicillin-resistant non- S. aureus staphylococci-MRNAS of veal calves were studied. MRNAS were highly multi-resistant and carried a broad range of resistance-genes. MRNAS carried typical Staphylococcus aureus virulence factors. MRNAS may represent a reservoir of antimicrobial resistance and virulence genes. Abstract: In this study we determined whether methicillin-resistant non- Staphylococcus aureus (MRNAS) from veal calves may be a potential reservoir of antimicrobial-resistance and virulence genes. Fifty-eight MRNAS were studied by means of DNA-microarray and PCR for detection of antimicrobial resistance and virulence genes. The isolates carried a variety of antimicrobial-resistance genes [ aacA-aphD, aadD, aph3, aadE, sat, spc, ampA, erm (A), erm (B), erm (C), erm (F), erm (T), lnu (A), msr (A)- msr (B), vga (A), mph (C), tet (K), tet (M), tet (L), cat, fexA, dfrA, dfrD, dfrG, dfrK, cfr, fusB, fosB, qacA, qacC, merA-merB ]. Some isolates carried resistance genes without showing the corresponding resistance phenotype. Most MRNAS carried typical S. aureus virulence factors like proteases ( sspP ) and enterotoxins ( seg ) genes. Most Staphylococcus epidermidis isolates carried the arginine catabolic element, and nearly 40% of the Staphylococcus sciuri isolates carried leukocidins, and/or fibronectin-binding protein genes. MRNAS were highly multi-resistant and represent an important reservoir of antimicrobial resistance and virulence genes.