Sea urchin (Strongylocentrotus intermedius) polysaccharide enhanced BMP-2 induced osteogenic differentiation and its structural analysis. (April 2015)

• Record Type: Journal Article
• Authors: Yang, Jingfeng; Yi, Meng; Pan, Jinfeng; Zhao, Jun; Sun, Liming; Lin, Xinping; Cao, Yuegang; Huang, Lu; Zhu, Beiwei; Yu, Chenxu
• Is Part Of: Journal of functional foods. Volume 14(2015)
• Page Start: 519
• Page End: 528
• Publication Date: 2015-04
• Subjects: Bone -- Regeneration -- Alkaline phosphatase -- C2C12; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2015.02.025
• Languages: English
• ISSNs: 1756-4646

Abstract

Highlights: A non-sulphate glucomannan (SUP) was isolated from Strongylocentrotus intermedius . SUP remarkably promoted the BMP-2 activity and induced osteoblast differentiation. The molecular structure of SUP was elucidated by two-dimensional NMR spectrum. Sulphate group not essential for polysaccharide enhances osteogenic differentiation. Abstract: A new sea urchin polysaccharide (SUP) was extracted from the gonad of sea urchin ( Strongylocentrotus intermedius ), and its bioactivity to synergistically enhance the functionality of bone morphogenetic protein-2 (BMP-2) induced osteoblastic differentiation was characterized. Results showed that the SUP is homogenous with an average molecular weight of 4.4 kDa, and it is mainly composed of mannose with a small number of glucose and N-acetylglucosamine, with a backbone of alternating 1, 4-D-Man and 1, 3-D-Man residues. The SUP was shown to synergistically enhance BMP-2 induced osteoblastic differentiation in C2C12 myoblast cells, evidenced by a significant increase in the activity of alkaline phosphatase (ALP), when the concentration of the SUP was higher than 10 µg/mL. The elevated ALP activity was also confirmed by histochemical staining of C2C12 myoblast cells. These results suggest that the SUP can promote bone regeneration and may serve as a functional food supplement for bone health.