Hematopoietic activity in putative mouse primordial germ cell populations. (May 2015)

• Record Type: Journal Article
• Authors: Scaldaferri, Maria Lucia; Klinger, Francesca Gioia; Farini, Donatella; Di Carlo, Anna; Carsetti, Rita; Giorda, Ezio; De Felici, Massimo
• Is Part Of: Mechanisms of development. Volume 136(2015)
• Page Start: 53
• Page End: 63
• Publication Date: 2015-05
• Subjects: Primordial germ cells -- Hematopoiesis -- Germline -- Allantois -- Oct3/4; Developmental biology -- Periodicals; Molecular biology -- Periodicals; Developmental Biology -- Periodicals; Molecular Biology -- Periodicals; Biologie du développement -- Périodiques; Biologie moléculaire -- Périodiques; Developmental biology; Molecular biology; Periodicals; Electronic journals; 571.8
• DOI: 10.1016/j.mod.2015.02.002
• Languages: English
• ISSNs: 0925-4773

Abstract

Highlights: Heterogeneous expression of the GOF-18ΔPE-GFP Oct3/4 transgene in putative PGCs. Putative PGCs express several genes of hematopoietic precursors. A subset of the putative PGCs (Oct3/4-GFP weak CD-34 weak/high ) possesses hematopoietic CFU activity. A subset of putative PGCs is not definitively germline determined. Abstract: In the present paper, starting from the observation of heterogeneous expression of the GOF-18ΔPE-GFP Pou5f1 ( Oct3/4 ) transgene in putative mouse PGC populations settled in the aorta–gonad–mesonephros (AGM) region, we identified various OCT3/4 positive populations showing distinct expression of PGC markers (BLIMP-1, AP, TG-1, STELLA) and co-expressing several proteins (CD-34, CD-41, FLK-1) and genes ( Brachyury, Hox-B4, Scl/Tal-1 and Gata-2 ) of hematopoietic precursors. Moreover, we found that Oct3/4-GFP weak CD-34 weak/high cells possess robust hematopoietic colony forming activity (CFU) in vitro. These data indicate that the cell population usually considered PGCs moving toward the gonadal ridges encompasses a subset of cells co-expressing several germ cell and hematopoietic markers and possessing hematopoietic activity. These results are discussed within of the current model of germline segregation.