Investigation of the functional properties and subcellular localization of alpha human and rainbow trout estrogen receptors within a unique yeast cellular context. Issue 149 (May 2015)

• Record Type: Journal Article
• Authors: Le Grand, Adélaïde; Bouter, Anthony; Couturier, Anne; Mulner-Lorillon, Odile; Le Goff, Xavier; Chesnel, Franck; Sire, Olivier; Le Tilly, Véronique
• Is Part Of: Journal of steroid biochemistry and molecular biology. Issue 149(2015)
• Page Start: 17
• Page End: 26
• Publication Date: 2015-05
• Subjects: E2 17β-estradiol -- ES estrogens -- ER estrogen receptor -- ERE estrogen response element -- LBD ligand-binding domain -- DBD DNA-binding domain -- EREcs consensus estrogen response element -- hERα human estrogen receptor alpha subtype -- rtERαS short isoform of alpha subtype of rainbow trout estrogen receptor -- MCF-7 human breast adenocarcinoma cell line -- AF-1 and AF-2 trans-activation functions 1 and 2 -- RAL raloxifene -- DES diethylstilbestrol -- 4-OHT 4-hydroxytamoxifen -- ICI ICI 182-780 -- rtvtgERE ERE found in the intergenic region of the rainbow trout vitellogenin gene -- GFP green fluorescent protein -- ONPG ortho-nitrophenylgalactoside -- EC50 effective concentration for half maximal response -- SHM syrian hamster myometrium -- COS-1 monkey kidney fibroblast cell line; Estrogen receptor -- Ligand binding -- Subcellular localization -- Transcriptional activity -- Confocal fluorescence microscopy; Steroid hormones -- Periodicals; Biochemistry -- Periodicals; Hormones -- Periodicals; Molecular Biology -- Periodicals; Hormones stéroïdes -- Périodiques; Steroid hormones; Periodicals; 572.579
• DOI: 10.1016/j.jsbmb.2015.01.007
• Languages: English
• ISSNs: 0960-0760

Abstract

Highlights: Sub-cellular localization of hERα and rtERαS expressed in a same cellular context. Effects of different estrogens on functional properties of hERα and rtERαS . Model assessing for the ligand-independent transcriptional activity of rtERαS . Abstract: Estrogens are steroid hormones that play a pivotal role in growth, differentiation and function of reproductive and non-reproductive tissues, mediated through estrogen receptors (ERs). Estrogens are involved in different genomic and non-genomic cell signaling pathways which involve well-defined subcellular ER localizations. Thus, ER activity results from complex interplays between intrinsic binding properties and specific subcellular localization. Since these two factors are deeply intricate, we carried out, in a unique yeast cell context, a comparative study to better understand structure/function/subcellular distribution relationships. This was carried out by comparing two ERs: the human ER α subtype (hERα) and the short form of the α isoform of the rainbow trout ER (rtERαS ). Their distinct binding properties to agonist and antagonist ligands and subcellular localizations were characterized in Saccharomyces cerevisiae yeast cells. An unexpected partial agonistic effect of ICI 182-780 was observed for rtERαS . Concomitant to distinct binding properties, distinct subcellular localizations were observed before and after ligand stimulation. Due to the unique cell context, the link between ERs intrinsic binding properties and subcellular localizations is partly unveiled and issues are hypothesized based on the role of cytoplasmic transient complexes which play a role in the ER cytoplasmic/nuclear partition, which in turn is critical for the recruitment of co-regulators in the nucleus.