One-step, visual and sensitive detection of phorate in blood based on a DNA–AgNC aptasensor. (28th March 2018)

• Record Type: Journal Article
• Authors: Li, Xingmei; Shi, Jian; Chen, Ceng; Li, Weichen; Han, Leiming; Lan, Lingmei; Guo, Yadong; Chang, Yunfeng; Cai, Jifeng; Ding, Yanjun
• Is Part Of: New journal of chemistry. Volume 42:Number 8(2018)
• Page Start: 6293
• Page End: 6298
• Publication Date: 2018-03-28
• Subjects: Chemistry -- Periodicals; Chimie -- Périodiques; 540
• DOI: 10.1039/c8nj00958a
• Languages: English
• ISSNs: 1144-0546

Abstract

Abstract : A one-step, visual and sensitive aptasensor based on DNA–AgNCs can be used to detect phorate in blood. Abstract : The ingestion of organophosphate (Op) pesticides for attempting suicide has been a serious health issue recently. Phorate, a class of Op, has attracted attention in this regard and so the rapid diagnosis and detection of phorate is crucial for human health. In this study, a one-step and visual method for the rapid determination of phorate via an aptasensor based on DNA-templated silver nanoclusters (DNA–AgNCs) has been presented. The designed DNA–AgNC nanoprobe, containing a special structure with an intercalated aptamer, shows excellent stability. Introducing phorate to the system easily triggers the aggregation of DNA–AgNCs, producing a brown-to-colorless color change in the solution in only 6 minutes. Under the optimal conditions, the aptasensor can allow for the determination of phorate in the concentration range of 0–25 μg mL −1, using two linear equations: y = −2.308 x + 0.996 ( R 2 = 0.977) for 0–0.125 μg mL −1 and y = −0.020 x + 0.664 ( R 2 = 0.971) for 0.125–25 μg mL −1 . The estimated detection limit of phorate was 0.012 ng mL −1 . Moreover, the practicability of this proposed method was also validated by analyzing phorate-spiked human blood samples and the results were in agreement with those from gas chromatography/mass spectrometry (GC/MS) analysis. Therefore, this fabricated aptasensor is suitable for sensing phorate in complex biological samples, and shows high potential for clinical diagnosis or forensic toxicology analysis.