In situ simultaneous profiling of phosphorylation and ubiquitination by single excitation-duplexed luminescence resonance energy transfer. Issue 29 (26th March 2018)

• Record Type: Journal Article
• Authors: Ma, Fengjiao; Zhang, Lei; Wong, Bernardino J. Córdova; Lei, Jianping
• Is Part Of: Chemical communications. Volume 54:Issue 29(2018)
• Page Start: 3648
• Page End: 3651
• Publication Date: 2018-03-26
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/c8cc01494a
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : A dual LRET system is developed for the in situ simultaneous profiling of phosphorylation and ubiquitination on a specific protein upon 980 nm excitation. Abstract : A dual luminescence resonance energy transfer system is developed based on aptamer-functionalized upconversion nanoparticles as the energy donor and a synthetic dye derivative as the acceptor, which can be applied in the simultaneous profiling and relative quantification of phosphorylation and ubiquitination on a specific protein, even at low levels of protein expression.