Nanodiamond-based layer-by-layer nanohybrids mediate targeted delivery of miR-34a for triple negative breast cancer therapy. Issue 25 (12th April 2018)

• Record Type: Journal Article
• Authors: Xia, Yang; Deng, Xiongwei; Cao, Minjun; Liu, Sha; Zhang, Xiaofei; Xiao, Xiangqian; Shen, Sisi; Hu, Qin; Sheng, Wang
• Is Part Of: RSC advances. Volume 8:Issue 25(2018)
• Page Start: 13789
• Page End: 13797
• Publication Date: 2018-04-12
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/c8ra00907d
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : The construction of nanodiamond-based layer-by-layer nanohybrids. Abstract : Triple-negative breast cancer (TNBC) is the most lethal subtype of breast cancer and significantly associated with poor prognosis and high risk of recurrence. miR-34a has been identified as a potent tumor suppressor whose expression is dramatically downregulated in TNBC. Currently, rectification of miRNA abnormality serves as a novel tumor therapeutic strategy. miR-34a is thus used as powerful antitumor agent for miRNA-based therapy against TNBC. However, miRNA-based antitumor therapy is challenged by effective targeted delivery of miRNA. In the present study, nanodiamond (ND), protamine (PS) and folic acid (FA) were used to construct ND-based layer-by-layer nanohybrids through a self-assembly approach for targeted miR-34a delivery in TNBC cells and xenograft TNBC tumors. We found that the targeted delivery of miR-34a remarkably suppressed cell proliferation, migration and induced the apoptosis of TNBC cells in vitro and inhibited tumor growth in vivo via down-regulating Fra-1 expression. The data suggest a great potential of ND-based nanohybrids for targeted intratumoral delivery of miR-34a for TNBC therapy.