Native RNA-Sequencing Throws its Hat into the Transcriptomics Ring. Issue 4 (April 2018)

• Record Type: Journal Article
• Authors: Hussain, Shobbir
• Is Part Of: Trends in biochemical sciences. Volume 43:Issue 4(2018)
• Page Start: 225
• Page End: 227
• Publication Date: 2018-04
• Subjects: nanopore sequencing -- Oxford Nanopore Technologies -- ONT -- MinION -- RNA-Seq -- alternative splicing -- epitranscriptome -- epitranscriptomics; Biochemistry -- Periodicals; 572
• DOI: 10.1016/j.tibs.2018.02.007
• Languages: English
• ISSNs: 0968-0004

Abstract

Abstract : De novo sequence-level surveys of transcriptomes have previously relied on sequencing via a DNA intermediate. While such methods can yield massive data sets, various problems mean that these do not always accurately reflect the true innate composition of transcriptomes. Enter Garalde et al ., who present for the first time highly parallel native RNA-Sequencing (RNA-seq), with potentially disruptive future-implications for the transcriptomics field.