Characterization of antioxidant and angiotensin-converting enzyme inhibitory peptides derived from cauliflower by-products by multidimensional liquid chromatography and bioinformatics. (May 2018)

• Record Type: Journal Article
• Authors: Montone, Carmela Maria; Capriotti, Anna Laura; Cavaliere, Chiara; La Barbera, Giorgia; Piovesana, Susy; Zenezini Chiozzi, Riccardo; Laganà, Aldo
• Is Part Of: Journal of functional foods. Volume 44(2018)
• Page Start: 40
• Page End: 47
• Publication Date: 2018-05
• Subjects: ABTS 2, 2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) -- ACE angiotensin-converting enzyme -- AO antioxidant -- DPPH 2, 2-diphenyl-1-picrylhydrazyl -- DTT dithiothreitol -- EDTA ethylenediaminetetraacetic acid -- HHL N-hippuryl-L-histidyl-L-leucine -- SDS sodium dodecyl sulphate -- SPE solid phase extraction -- TCA trichloroacetic acid -- TFA trifluoroacetic acid; Peptidomics -- Cauliflower waste -- Antioxidant peptides -- ACE-inhibitory peptides; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2018.02.022
• Languages: English
• ISSNs: 1756-4646

Abstract

Graphical abstract: Highlights: Cauliflower by-products are a potential source of value-added compounds. Semi-industrial scale up strategy for production of bioactive peptides. Multidimensional liquid chromatography for purification of bioactive peptides. Four novel bioactive peptides identified from cauliflower waste. Abstract: In the present paper, bioactive peptides from cauliflower by-products (leaves and stems) were investigated. Alcalase® protein hydrolysis time and pH conditions were optimized, then the hydrolysates were fractionated by preparative RP-HPLC into 12 fractions. Each fraction was tested for the ABTS (2, 2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)), and DPPH (2, 2-diphenyl-1-picrylhydrazyl) radical scavenging activity and for ACE (angiotensin-converting enzyme) inhibitor activity. The peptides in the most active fractions were identified by peptidomics technologies and screened for bioactivity by the use of bioinformatics. For ACE-inhibitor activity, two peptides were synthetized, APYDPDWYYIR and SKGFTSPLF, which provided an EC50 value of 2.59 μmol L −1 and 15.26 μmol L −1, respectively. For the ABTS radical scavenging activity, SKGFTSPLF and LDDPVFRPL were tested, and provided an EC50 of 10.35 μmol L −1 and 8.29 μmol L −1, respectively. For the DPPH radical scavenging activity, SKGFTSPLF and LRAPPGWTGR were tested and provided an EC50 of 8.2 μmol L −1 and 5.26 μmol L −1, respectively.