Human mesenchymal stem cell viability, proliferation and differentiation potential in response to ceramic chemistry and surface roughness. Issue 5 (June 2015)

• Record Type: Journal Article
• Authors: Mazón, Patricia; García-Bernal, David; Meseguer-Olmo, Luis; Cragnolini, Francesca; De Aza, Piedad N.
• Is Part Of: Ceramics international. Volume 41:Issue 5(2015)Part A
• Page Start: 6631
• Page End: 6644
• Publication Date: 2015-06
• Subjects: A. Powder solid-state reaction -- B. Surfaces -- D. Apatite -- E. Biomedical applications -- Bone regeneration; Ceramics -- Periodicals; Céramique industrielle -- Périodiques; Ceramics; Periodicals; Electronic journals; 666
• DOI: 10.1016/j.ceramint.2015.01.110
• Languages: English
• ISSNs: 0272-8842

Abstract

Abstract: We investigated the effect of the ceramic chemistry and surface roughness of pure α-tricalcium phosphate, and also αTCP doped with either 1.5 wt% or 3.0 wt% of dicalcium silicate (C2 S), on the response of adult human mesenchymal stem cells ( ah MSCs). A h MSCs were plated onto ceramic discs, prepared by a solid-state reaction. After being sintered, some samples were polished up to 1 μm, while others were kept as manufactured, which resulted in two surface roughness grades. Viability, proliferation and osteoinductive capacity were determined following various incubation periods. The results showed a non-cytotoxic effect after an indirect apoptosis test. Cell adhesion and proliferation were surface roughness-sensitive and increased proportionally to the roughness of materials. These observations became more evident in the unpolished αTCP ceramic doped with 1.5 wt% C2 S, which induced osteoblastic differentiation as a result of the roughness and increased concentration of the C2 S solid solution in αTCP.