Rutin ameliorates cyclophosphamide induced oxidative stress and inflammation in Wistar rats: Role of NFκB/MAPK pathway. (25th April 2015)

• Record Type: Journal Article
• Authors: Nafees, Sana; Rashid, Summya; Ali, Nemat; Hasan, Syed Kazim; Sultana, Sarwat
• Is Part Of: Chemico-biological interactions. Volume 231(2015)
• Page Start: 98
• Page End: 107
• Publication Date: 2015-04-25
• Subjects: BSA bovine serum albumin -- CAT catalase -- CDNB 1-chloro 2, 4-dinitrobenzene -- COX-2 cycloxygenase-2 -- CP cyclophosphamide -- DAB-3 3-diaminobenzidine -- DTNB-5 5′-dithio bis-[2-nitrobenzoic acid] -- EDTA ethylene diamine tetra acetic acid -- GPx glutathione peroxidase -- GR glutathione reductase -- GSH reduced glutathione -- GSSG oxidized glutathione -- IL-6 Interleukin-6 -- i-NOS inducible nitric oxide synthase -- LPO lipidperoxidation -- MAPKs mitogen-activated protein kinases -- MDA malondialdehyde -- NADPH reduced nicotinamide adenine dinucleotide phosphate -- NFκB nuclear factor kappa B -- NO nitric oxide -- NOS nitric oxide synthase -- ROS reactive oxygen species -- TNF-α Tumor Necrosis Factor Alpha -- XO xanthine oxidase; Cyclophosphamide -- Rutin -- Oxidative stress -- Inflammation -- NFκB -- MAPK; Biochemistry -- Periodicals; Toxicological chemistry -- Periodicals; Biochemistry -- Periodicals; Biologie moléculaire -- Périodiques; Biochimie -- Périodiques; Toxicologie biochimique -- Périodiques; 572
• DOI: 10.1016/j.cbi.2015.02.021
• Languages: English
• ISSNs: 0009-2797

Abstract

Graphical abstract: Highlights: Role of rutin in alleviating oxidative stress in liver. Role of rutin in targeting hepatic injury by inhibition of inflammation in liver tissue. Role of rutin in suppressing histopathological changes induced by cyclophosphamide induced hepatotoxicity. Abstract: Cyclophosphamide is a potent anticancer agent. However its clinical use is restricted because of its marked organ toxicity associated with increased oxidative stress and inflammation. The present study was designed to demonstrate the protective effects of rutin, a naturally occurring bioflavonoid against the hepatotoxicity induced by CP. Rats were subjected to oral pretreatment of rutin (50 and 100 mg/kg b wt) against hepatotoxicity induced by i.p. injection of CP (150 mg/kg b wt) and were sacrificed after 24 h. Hepatoprotective effects of rutin were associated with upregulation of antioxidant enzyme activities and down regulation of serum toxicity markers. Rutin was able to down regulate the levels of inflammatory markers like TNF-α, IL-6 and expressions of p38-MAPK, NFκB, i-NOS and COX-2. Histopathological changes further confirmed the biochemical and immunohistochemical results showing that CP caused significant structural damage to liver which were reversed by pretreatment of rutin. Therefore, our study revealed that rutin may be a promising modulator in attenuating CP induced oxidative stress, inflammation and hepatotoxicity via targeting NFκB and MAPK pathway.