Structure of a new pseudaminic acid-containing capsular polysaccharide of Acinetobacter baumannii LUH5550 having the KL42 capsule biosynthesis locus. (30th April 2015)

• Record Type: Journal Article
• Authors: Senchenkova, Sof'ya N.; Popova, Anastasiya V.; Shashkov, Alexander S.; Shneider, Mikhail M.; Mei, Zhu; Arbatsky, Nikolay P.; Liu, Bin; Miroshnikov, Konstantin A.; Volozhantsev, Nikolay V.; Knirel, Yuriy A.
• Is Part Of: Carbohydrate research. Volume 407(2015)
• Page Start: 154
• Page End: 157
• Publication Date: 2015-04-30
• Subjects: Acinetobacter baumannii -- Capsular polysaccharide structure -- Polysaccharide gene locus -- Pseudaminic acid; Carbohydrates -- Periodicals; Chemistry, Organic -- Periodicals; Biochemistry -- Periodicals; Carbohydrates -- Periodicals; Chimie organique -- Périodiques; Glucides -- Périodiques; Biochemistry; Carbohydrates; Chemistry, Organic; Periodicals; Electronic journals; 507.78
• DOI: 10.1016/j.carres.2015.02.006
• Languages: English
• ISSNs: 0008-6215

Abstract

Abstract: The capsular polysaccharide from Acinetobacter baumannii LUH5550 was studied by 1D and 2D 1 H and 13 C NMR spectroscopy. The following structure of the branched trisaccharide repeating unit was established: where Pse5Ac7 R Hb indicates 5-acetamido-3, 5, 7, 9-tetradeoxy-7-[( R )-3-hydroxybutanoylamino]-l - glycero -l - manno -non-2-ulosonic acid. The genes in the capsule biosynthesis locus designated KL42 are consistent with the structure established. Graphical abstract: Highlights: Capsular polysaccharide was isolated from Acinetobacter baumannii LUH5550. The polysaccharide contains 5-N-acetyl-7-N-[( R )-3-hydroxybutanoyl]pseudaminic acid. Structure of the polysaccharide was established by NMR and chemical approaches. Capsule biosynthesis locus was analyzed and found to match the structure established.