Placental function and structure at term is altered in broodmares fed with cereals from mid-gestation. (April 2018)

• Record Type: Journal Article
• Authors: Robles, M.; Peugnet, P.; Dubois, C.; Piumi, F.; Jouneau, L.; Bouchez, O.; Aubrière, M.C.; Dahirel, M.; Aioun, J.; Wimel, L.; Couturier-Tarrade, A.; Chavatte-Palmer, P.
• Is Part Of: Placenta. Volume 64(2018)
• Page Start: 44
• Page End: 52
• Publication Date: 2018-04
• Subjects: Placenta -- Periodicals; Reproduction -- Periodicals; Placenta -- Periodicals; Placenta -- Périodiques; Reproduction -- Périodiques; 612.63
• DOI: 10.1016/j.placenta.2018.02.003
• Languages: English
• ISSNs: 0143-4004

Abstract

Abstract: Introduction: Feeding pregnant broodmares with cereal concentrates has been shown to increase maternal insulin resistance and affect foal metabolism in the short and long-term. These effects are likely to be mediated by the placenta. Here, we investigated feto-placental biometry and placental structure and function at term in mares fed with or without cereals concentrates. Material and methods: From 7 months of gestation, 22 multiparous mares were fed forage only (group F (n = 12)) or received forage and cracked barley (group B (n = 10)) until foaling. Foals and placentas were weighed and placental samples were collected above the umbilical cord insertion at birth. Placental histological structure was studied by stereology. A RNAseq analysis was performed on 9 placentas of each group. Enrichment of gene sets was analysed using the Gene Set Enrichment Analysis (GSEA) software using the KEGG and GO databases. Results: No difference in feto-placental biometry was observed between groups. The volume of microcotyledonary vessels was decreased in B placentas and the vascular wall of allantoic arterioles was thickened. Gene sets involved in neutral amino acids, folate and anions transport and fatty acids, cholesterol and folate degradation were down-regulated while gene sets involved in RNA expression, inflammation and vascularisation were up-regulated in B placentas. Conclusion: Feeding pregnant mares with concentrates from mid-gestation alters the placental function and structure as observed in other species in cases of maternal insulin resistance. Highlights: Feeding cereals to broodmares in late gestation alters the vasculature development of placentas. Feeding cereals to broodmares in late gestation alters the placental transcriptome. Insulin resistance in late gestation in the horse alters the placental structure and function as observed in obese women.