Emodin induces neurite outgrowth through PI3K/Akt/GSK-3β-mediated signaling pathways in Neuro2a cells. (19th February 2015)

• Record Type: Journal Article
• Authors: Park, Shin-Ji; Jin, Mei Ling; An, Hyun-Kyu; Kim, Kyoung-Sook; Ko, Min Jung; Kim, Cheol Min; Choi, Young Whan; Lee, Young-Choon
• Is Part Of: Neuroscience letters. Volume 588(2015)
• Page Start: 101
• Page End: 107
• Publication Date: 2015-02-19
• Subjects: Emodin -- Neurite outgrowth -- Neuronal differentiation -- PI3K/Akt/GSK-3β pathway -- Neuro2a cells; Neurology -- Periodicals; Neurology -- Periodicals; Research -- Periodicals; Neurologie -- Périodiques; Neuroanatomie -- Périodiques; Neuropharmacologie -- Périodiques; Neurophysiologie -- Périodiques; Neurology; Periodicals; Electronic journals; 617.48
• DOI: 10.1016/j.neulet.2015.01.001
• Languages: English
• ISSNs: 0304-3940

Abstract

Highlights: We isolated emodin from roots of dried Polygonum multiflorum . Treatment of emodin induced neurite outgrowth in Neuro2a cells. Emodin-induced neurite outgrowth is associated with PI3K/Akt/GSK-3β signaling pathway in Neuro2a cells. Emodin-induced neurite outgrowth was significantly suppressed by PI3K/Akt inhibitor LY294002. Abstract: In this study, a neurite outgrowth-inducing substance was isolated from the ethylacetate extract of the Polygonum multiflorum roots and identified as emodin by gas–liquid chromatography–mass spectrometry and 1 H NMR and 13 C NMR. Emodin displayed remarkable neurite outgrowth-inducing activity in Neuro2a cells, as demonstrated by morphological changes and immunocytochemistry for class III β-tubulin. Emodin exhibited a stronger neutrophic activity than retinoic acid (RA) known as inducer of neurite outgrowth in Neuro2a cells. Emodin treatment resulted in marked increases in phosphorylation of Akt a direct downstream signaling molecule of phosphatidylinositol 3-kinase (PI3K), but upstream of glycogen synthase kinase-3β (GSK-3β) and cAMP response element-binding protein (CREB). These augmentations and neurite-bearing cells induced by emodin were remarkably reduced by the addition of PI3K inhibitor LY294002. These results demonstrate that emodin induces neuronal differentiation of Neuro2a cells via PI3K/Akt/GSK-3β pathway.