MiR-501 is upregulated in cervical cancer and promotes cell proliferation, migration and invasion by targeting CYLD. (1st April 2018)

• Record Type: Journal Article
• Authors: Sanches, Jaceline Gislaine Pires; Xu, Yunchao; Yabasin, Iddrisu Baba; Li, Min; Lu, Ying; Xiu, Xiaoxin; Wang, Lu; Mao, Limin; Shen, Jie; Wang, Bo; Hou, Li; Ju, Jingfang; Zhao, Junjun; Song, Bo
• Is Part Of: Chemico-biological interactions. Volume 285(2018)
• Page Start: 85
• Page End: 95
• Publication Date: 2018-04-01
• Subjects: miR-501 -- Cervical cancer -- CYLD -- Cell proliferation -- Invasion; miR-501 microRNA-501-5p -- CYLD Cylindromatosis -- NC negative Control -- miR-501 miR-501 mimic -- 501i miR-501 inhibitor -- siCYLD siRNA against CYLD -- CCK-8 Cell Counting Kit 8 -- FIGO International Federation of Gynecology and Obstetrics -- PBS Phosphate buffer solution -- SDS-PAGE Sodium dodecyl sulfate-poly acrylamide gel electrophoresis -- qRT-PCR quantitative real time-polymerase chain reaction -- FFPE Formalin-fixed and paraffin-embedded -- IHC Immunohistochemistry -- DAB Diaminobenzidine -- DPX p-xylene-bis-pyridinium bromide -- PI Propedium iodide; Biochemistry -- Periodicals; Toxicological chemistry -- Periodicals; Biochemistry -- Periodicals; Biologie moléculaire -- Périodiques; Biochimie -- Périodiques; Toxicologie biochimique -- Périodiques; 572
• DOI: 10.1016/j.cbi.2018.02.024
• Languages: English
• ISSNs: 0009-2797

Abstract

Abstract: Background: Cervical cancer is the common gynecological deadly malignancy worldwide. Here we attempted to evaluate the effects and mechanisms of microRNA-501-5p (miR-501) on the cell proliferation, migration, invasion and the clinical significance in the cervical cancer. Methods: Cervical cancer HeLa cells were transfected with miR-501 mimic or inhibitor or siRNA against Cylindromatosis (CYLD) using Lipofectamine 2000. miR-501 expression was assessed in HeLa cells and cervical cancer specimens by real-time qRT-PCR. The functional roles of miR-501 were determined by CCK-8, colony formation, scratch wound healing and transwell assays. The apoptosis rate was detected by flow cytometry assay. CYLD, BCL-2, BAX, NF- κ B p65 and phosphorylated p65 (p-p65) proteins were examined by Western blotting. CYLD expression was evaluated by immunohistochemistry in cervical cancer tissues. Results: miR-501 was upregulated, whereas CYLD protein was downregulated in cervical cancer tissues compared to normal cervical tissues. miR-501 overexpression and CYLD protein downregulation were positively correlated with poor differentiation, tumor size, International Federation of Gynecology and Obstetrics (FIGO) stage and lymph node metastasis. CYLD was downregulated by miR-501 at both mRNA and protein levels in HeLa cells. miR-501 promoted cell proliferation, migration and invasion in cervical cancer, while inhibited the apoptosis. This is possibly due to the downregulation of CYLD and subsequent activation of NF-κB p65. Conclusions: miR-501 upregulation and CYLD downregulation are associated with the development and progression of cervical cancer. miR-501 promotes cervical cancer cell proliferation, migration and invasion possibly via downregulating CYLD and subsequently activating NF-κB p65. miR-501 might be a potential therapeutic target for cervical cancer. Highlights: miR-501 promotes the development and progression of cervical cancer. miR-501 induces cell proliferation, migration, invasion and inhibits apoptosis. CYLD is downregulated by miR-501 at post-transcriptional level in cervical cancer. CYLD suppresses the development and progression of cervical cancer. miR-501 might be a potential therapeutic target for cervical cancer.