Biliary excretion of tetrodotoxin in the cultured pufferfish Takifugu rubripes juvenile after intramuscular administration. (January 2015)

• Record Type: Journal Article
• Authors: Matsumoto, Takuya; Kiriake, Aya; Ishizaki, Shoichiro; Watabe, Shugo; Nagashima, Yuji
• Is Part Of: Toxicon. Volume 93(2015)
• Page Start: 98
• Page End: 102
• Publication Date: 2015-01
• Subjects: Tetrodotoxin -- Pufferfish Takifugu rubripes -- Juvenile -- Biliary excretion -- Gallbladder -- Intramuscular administration -- Accumulation -- Toxification; LC-MS/MS liquid chromatography tandem mass spectrometry -- TTX tetrodotoxin -- STX saxitoxin -- PSTBP pufferfish saxitoxin and tetrodotoxin binding protein; Toxins -- Periodicals; Venom -- Periodicals; 615.9
• DOI: 10.1016/j.toxicon.2014.11.227
• Languages: English
• ISSNs: 0041-0101

Abstract

Abstract: Marine pufferfish of the family Tetraodontidae accumulate a considerable amount of tetrodotoxin (TTX), mainly in the liver and ovary. The detailed distribution of TTX in pufferfish body tissues, however, remains poorly understood. Here we investigated the tissue distribution and biliary excretion of TTX in cultured pufferfish Takifugu rubripes juveniles (6-month-old, 81.5 ± 2.0 g body weight) for 24 h after intramuscular administration of 0.25 μg TTX/g body weight into the caudal muscle. The blood TTX concentration was 0.53 ± 0.15 μg/mL at 1 h, and gradually decreased to 0.05 ± 0.01 μg/mL at 24 h after administration ( p < 0.05). The TTX concentration in the liver declined from 1.59 ± 0.10 μg/g at 1 h to 0.48 ± 0.21 μg/g at 24 h ( p < 0.05). In contrast, the TTX concentration in the skin increased from 0.27 ± 0.04 μg/g at 1 h to 0.48 ± 0.08 μg/g at 24 h ( p < 0.05). The concentration of TTX in the bile remarkably increased from 0.08 ± 0.03 μg/mL at 1 h to 0.39 ± 0.05 μg/mL at 8 h ( p < 0.05) and remained at almost the same level at 24 h. These findings indicate that TTX was excreted from the liver into the gallbladder bile in the pufferfish T. rubripes juveniles. Highlights: We investigated the tissue distribution and biliary excretion of tetrodotoxin in cultured pufferfish juveniles. The test fish received administration of tetrodotoxin into the caudal muscle. Tetrodotoxin administered was initially concentrated into the liver, and then transferred to the skin over time up to 24 h. The tetrodotoxin concentration in the bile remarkably increased over time after administration.