Genetic structure and historical demography of the blue swimming crab (Portunus pelagicus) from southeastern sea of China based on mitochondrial COI gene. (17th February 2018)

• Record Type: Journal Article
• Authors: Ren, Guijing; Miao, Guidong; Ma, Chunyan; Lu, Jianxue; Yang, Xiaolong; Ma, Hongyu
• Is Part Of: Mitochondrial DNA. Volume 29:Number 2(2018)
• Page Start: 192
• Page End: 198
• Publication Date: 2018-02-17
• Subjects: Portunus pelagicus -- population genetic structure -- historical demography -- COI gene -- phylogeney
• DOI: 10.1080/24701394.2016.1261855
• Languages: English
• ISSNs: 2380-2359

Abstract

Abstract: In this study, the population genetic structure and historical demography of the blue swimming crab, Portunus pelagicus, from southeastern sea of China were investigated using cytochrome c oxidase subunit I (COI) gene of mitochondrion. A total of 889 bp segment of COI gene was sequenced, which showed a high haplotype diversity (0.6833–0.8142) and low nucleotide diversity (0.0021–0.0034). Among 30 haplotypes defined in this study, one (H1) was the most dominant (47.7%) and shared by each locality, while the majority (23) were rare and only existed in one individual. The AMOVA analysis revealed a limited population genetic structure, which suggested a high level of gene flow along the distribution areas of China. This conclusion was supported by the pairwise F ST comparison values. The topology of the neighbour-joining tree constructed using 30 haplotypes from four localities presented two distinct clades (clade A and clade B). Meanwhile, three sequences of P. pelagicus downloaded from NCBI database showed a high-level divergence with the individuals collected in our study, which might form a new cryptical species. The individuals of clade B were cryptically embedded in the whole population, with a low frequency (7.7–24.2%), while clade A accounted for 75.8–92.3%. Neutrality tests and mismatch analyses suggested a late Pleistocene population expansion for both clade A (47, 000–66, 000 years ago) and clade B (74, 000–100, 000 years ago). This study should provide insight into phylogeny, population genetic structure, conservation genetics, and sustainable management of P. pelagicus .