Qualitative and quantitative detection of chicken deoxyribonucleic acid (DNA) in dry dog foods. (5th April 2018)

• Record Type: Journal Article
• Authors: Hołda, K.; Natonek‐Wiśniewska, M.; Krzyścin, P.; Głogowski, R.
• Other Names: Paßlack Nadine guestEditor.; Kröger Susan guestEditor.; Zentek Jürgen guestEditor.
• Is Part Of: Journal of animal physiology and animal nutrition. Volume 102(2018)Supplement 1
• Page Start: 37
• Page End: 42
• Publication Date: 2018-04-05
• Subjects: chicken DNA -- feed control -- pet food -- polymerase chain reaction; Animal nutrition -- Periodicals; Feeds -- Periodicals; 636.085
• DOI: 10.1111/jpn.12887
• Languages: English
• ISSNs: 0931-2439

Abstract

Summary: Chicken is a common protein source in pet foods and is concurrently listed among food allergens. Commercial over‐the‐counter (OTC) diets with an alternative animal protein source are considered suitable for dietary elimination trials by pet owners. The potential presence of undeclared chicken‐derived ingredients in these diets can compromise the outcome of the trial during the diagnosis of adverse food reactions. The aim of this study was to selectively verify the absence or presence of chicken DNA in 10 OTC dry canine foods, using qualitative and quantitative approaches. The method of identification of chicken‐derived protein was elaborated with the polymerase chain reaction (PCR) technology, whereas quantitative real‐time PCR was used for the quantitative assessment. In most of the analysed samples, the chicken DNA was detectable; however, the quantified amounts were predominantly low, although differences between batches were observed.