A histopathological classification system of Tyr:NRASQ61K murine melanocytic lesions: A reproducible simplified classification. (22nd December 2017)

• Record Type: Journal Article
• Authors: Sohier, Pierre; Legrand, Léa; Aktary, Zackie; Grill, Christine; Delmas, Véronique; Bernex, Florence; Reyes‐Gomez, Edouard; Larue, Lionel; Vergier, Béatrice
• Is Part Of: Pigment cell & melanoma research. Volume 31:Number 3(2018)
• Page Start: 423
• Page End: 431
• Publication Date: 2017-12-22
• Subjects: β‐catenin -- histopathology -- Ink4a -- melanocyte -- melanoma -- melanoma model -- Pten -- Ras -- transgenic mice; Melanoma -- Periodicals; Chromatophores -- Periodicals; Animal pigments -- Periodicals; 616.99477
• DOI: 10.1111/pcmr.12677
• Languages: English
• ISSNs: 1755-1471

Abstract

Summary: Genetically engineered mouse models offer essential opportunities to investigate the mechanisms of initiation and progression in melanoma. Here, we report a new simplified histopathology classification of mouse melanocytic lesions in Tyr::NRAS Q 61K derived models, using an interactive decision tree that produces homogeneous categories. Reproducibility for this classification system was evaluated on a panel of representative cases of murine melanocytic lesions by pathologists and basic scientists. Reproducibility, measured as inter‐rater agreement between evaluators using a modified Fleiss' kappa statistic, revealed a very good agreement between observers. Should this new simplified classification be adopted, it would create a robust system of communication between researchers in the field of mouse melanoma models.