Electron transfer rate analysis of a site-specifically wired copper oxidase. Issue 9 (19th February 2018)

• Record Type: Journal Article
• Authors: Schlesinger, Orr; Pasi, Mor; Dandela, Rambabu; Meijler, Michael M.; Alfonta, Lital
• Is Part Of: Physical chemistry chemical physics. Volume 20:Issue 9(2018)
• Page Start: 6159
• Page End: 6166
• Publication Date: 2018-02-19
• Subjects: Chemistry, Physical and theoretical -- Periodicals; 541.3
• DOI: 10.1039/c8cp00041g
• Languages: English
• ISSNs: 1463-9076

Abstract

Abstract : Electron transfer kinetic parameters of site-specifically wired copper oxidase were investigated. Abstract : Electron transfer kinetic parameters of site-specifically wired copper oxidase were investigated. The enzyme's orientation towards the electrode was controlled by incorporation of propargyl-l -lysine as a site-specific anchoring point. Herein, we demonstrate the importance of immobilization orientation and how it affects electron transfer efficiency and kinetics to each of the enzyme's two active sites.