A turn-on near-infrared fluorescence probe with aggregation-induced emission based on dibenzo[a, c]phenazine for detection of superoxide anions and its application in cell imaging. Issue 5 (12th February 2018)

• Record Type: Journal Article
• Authors: Yang, Ji; Liu, Xianglong; Wang, Honglei; Tan, Haoqi; Xie, Xiaoxu; Zhang, Xiao; Liu, Chenchen; Qu, Xue; Hua, Jianli
• Is Part Of: Analyst. Volume 143:Issue 5(2018)
• Page Start: 1242
• Page End: 1249
• Publication Date: 2018-02-12
• Subjects: Chemistry, Analytic -- Periodicals; 543
• DOI: 10.1039/c7an01860f
• Languages: English
• ISSNs: 0003-2654

Abstract

Abstract : A new turn-on near-infrared fluorescence probe (BDP ) based on dibenzo[ a, c ]phenazine for superoxide anion detection with AIE properties and a large Stokes shift was developed. Abstract : A new turn-on near-infrared fluorescence probe (BDP ) based on dibenzo[ a, c ]phenazine for superoxide anion detection with aggregation-induced emission properties as well as a desirable large Stokes shift was designed and synthesized. After BDP reacted with superoxide, the initial diphenyl-phosphinyl groups of BDP were cleaved, resulting in the production of the pyridinium modified fluorophore (BD ) with near-infrared emission. The fluorescent sensorBDP has a high selectivity for superoxide anions over some other intracellular ROSs, reductants, metal ions and amino acids. When HepG2 cells undergo apoptosis and inflammation, BDP is a good probe to keep track of the endogenous superoxide anion level by confocal laser scanning microscopic imaging.