A series of two-photon absorption pyridinium sulfonate inner salts targeting endoplasmic reticulum (ER), inducing cellular stress and mitochondria-mediated apoptosis in cancer cells. Issue 13 (14th March 2018)

• Record Type: Journal Article
• Authors: Hussain, Sajid; Du, Wei; Zhang, Mingzhu; Fang, Bin; Zhang, Guocui; Su, Rina; Nan, Keqian; Zhang, Qiong; Tian, Xiaohe; Tian, Yupeng; Chen, Yan
• Is Part Of: Journal of materials chemistry. Volume 6:Issue 13(2018)
• Page Start: 1943
• Page End: 1950
• Publication Date: 2018-03-14
• Subjects: Materials -- Periodicals; Chemistry, Analytic -- Periodicals; Biomedical materials -- Research -- Periodicals; 543.0284
• DOI: 10.1039/c8tb00173a
• Languages: English
• ISSNs: 2050-750X

Abstract

Abstract : DiphenthioER1 is a novel two-photon active pyridinium derivative that labels ER and causes nuclear misshaping and mitochondria-mediated apoptosis. Abstract : In this study, a two-photon activeDiphenthioER1 was screened from the four pyridinium sulfonate salt derivatives (TriphenER1-2 andDiphenthioER1-2 ) for imaging endoplasmic reticulum (ER) and tracking the dynamics of the ER morphology. The photophysical properties ofTriphenER1-2 andDiphenthioER1-2 were systemically investigated both experimentally and theoretically, revealing that they possess large Stokes shifts, and large two-photon absorption cross-sections from 163 GM to 2023 GM in the near infrared region using a Z-scan method by avoiding spontaneous fluorescence, deep tissue penetration, and low cell damage in living cells. Among them, theDiphenthioER1 compound was found to exhibit the highest cellular uptake ability and two-photon fluorescence signals using confocal microscopy.DiphenthioER1 successfully targeted the ER and induced ER-stress, subsequently nuclear misshaping and mitochondria-mediated apoptosis have been displayed. This study thus provides great insights into designing novel two-photon fluorescent materials with dual functionality and offers tools to understand the ER-stress related mechanism for cell and chemical biologists.