A fast responsive, highly selective and light-up fluorescent probe for the two-photon imaging of carboxylesterase in living cells. Issue 11 (1st March 2018)

• Record Type: Journal Article
• Authors: Wang, Jianguo; Chen, Qingqing; Tian, Na; Zhu, Wenping; Zou, Hang; Wang, Xuesong; Li, Xiaokang; Fan, Xiaolin; Jiang, Guoyu; Tang, Ben Zhong
• Is Part Of: Journal of materials chemistry. Volume 6:Issue 11(2018)
• Page Start: 1595
• Page End: 1599
• Publication Date: 2018-03-01
• Subjects: Materials -- Periodicals; Chemistry, Analytic -- Periodicals; Biomedical materials -- Research -- Periodicals; 543.0284
• DOI: 10.1039/c8tb00147b
• Languages: English
• ISSNs: 2050-750X

Abstract

Abstract : A fast responsive and two photon fluorescent probe (HCyNAc) for carboxylesterase (CaE) has been designed and used for the two-photon imaging of the endogenous CaE level in living HeLa cells under 800 nm NIR excitation. Abstract : A fast responsive and two photon fluorescent probe (HCyNAc) for carboxylesterase (CaE) has been designed based on the D–π–A structure of hemicyanine and naphthalene derivatives. After enzymatic reaction, HCyNAc enabled light-up fluorescence assay of CaE over other biologically-relevant species and enzymes, including ROS. Two-photon imaging of endogenous CaE was confirmed in HeLa cells using HCyNAc under 800 nm NIR excitation.