Alginate dialdehyde meets nylon membrane: a versatile platform for facile and green fabrication of membrane adsorbers. Issue 11 (1st March 2018)

• Record Type: Journal Article
• Authors: Khan, M. Kamran; Luo, Jianquan; Wang, Zhaoshuai; Khan, Rashid; Chen, Xiangrong; Wan, Yinhua
• Is Part Of: Journal of materials chemistry. Volume 6:Issue 11(2018)
• Page Start: 1640
• Page End: 1649
• Publication Date: 2018-03-01
• Subjects: Materials -- Periodicals; Chemistry, Analytic -- Periodicals; Biomedical materials -- Research -- Periodicals; 543.0284
• DOI: 10.1039/c7tb02966g
• Languages: English
• ISSNs: 2050-750X

Abstract

Abstract : Alginate dialdehyde, a biocompatible polymer, is used as an intermediate layer on a nylon membrane to readily fabricate different membrane adsorbers. Abstract : Alginate dialdehyde (ADA), a biocompatible polymer, was used as an intermediate layer on a nylon membrane to readily fabricate cation exchange (CEX), metal-affinity (Me-affinity), histidine-affinity (His-affinity) and peptide-affinity (Pep-affinity) membrane adsorbers without any organic solvent usage. All the membrane adsorbers exhibited a high selectivity in the fractionation of a IgG (immunoglobulin)/HSA (human serum albumin) mixture. Along with a high purity of 100%, a high IgG binding capacity of 30, 24, 21 and 28 mg mL −1 (membrane volume) was achieved by the CEX, Me-affinity, His-affinity and Pep-affinity membrane adsorbers in flow-through mode respectively, which is superior to those of the reported membrane adsorbers. Furthermore, the CEX and Pep-affinity adsorbers were tested for the separation of IgG from human plasma solution. The carboxylic groups along with the peptides on the Pep-affinity adsorber captured IgG synergistically with a higher recovery and purity (99% and 98.6%). This synergetic adsorber showed a q max and dynamic binding capacity (DBC) of 138.4 and 38.7 mg mL −1 respectively for IgG binding which was somewhat higher than that of the reported protein A agarose (having q max and DBC of 84 and 24.4 mg g −1 respectively). The present work indicated that the ADA layer not only activated the membrane surface to attach various adsorptive ligands under mild conditions, but also reduced non-specific adsorption. Due to the versatile linking function and green reaction conditions, the ADA coating on the nylon membrane is promising for the preparation of diverse membrane adsorbers.