Crystal structures of FMN‐bound and FMN‐free forms of dihydroorotate dehydrogenase from Trypanosoma brucei. Issue 4 (6th March 2018)

• Record Type: Journal Article
• Authors: Kubota, Tomomi; Tani, Osamu; Yamaguchi, Tomohiko; Namatame, Ichiji; Sakashita, Hitoshi; Furukawa, Koji; Yamasaki, Kazuhiko
• Is Part Of: FEBS open bio. Volume 8:Issue 4(2018)
• Page Start: 680
• Page End: 691
• Publication Date: 2018-03-06
• Subjects: apoform -- conformational flexibility -- flavin‐binding enzyme -- neglected tropical disease -- peptide flipping -- Trypanosoma brucei -- X‐ray crystallography; Molecular biology -- Periodicals; Cytology -- Periodicals; Life sciences -- Periodicals; Biological Science Disciplines -- Periodicals; Molecular Biology -- Periodicals; Cell Biology -- Periodicals; Cytology; Life sciences; Molecular biology; Periodicals; 572.805
• DOI: 10.1002/2211-5463.12403
• Languages: English
• ISSNs: 2211-5463

Abstract

Abstract : Dihydroorotate dehydrogenase (DHODH) is a flavin‐binding enzyme essential for pyrimidine biosynthesis, which converts dihydroorotate to orotate. Three‐dimensional structures of cytosolic DHODH of parasitic protozoa are of interest in drug discovery for neglected tropical diseases, especially because these enzymes possess significantly different structural and functional properties from the membrane‐associated human enzyme. The existing crystal structures of the flavin mononucleotide (FMN)‐bound DHODHs reveal a number of interactions stabilizing FMN. However, to understand the binding mechanism correctly, it is necessary to compare the structures of the FMN‐bound and FMN‐free forms, because the protein moiety of the former is not necessarily the same as the latter. Here, we prepared the FMN‐free DHODH of Trypanosoma brucei using an Escherichia coli overexpression system. Although this apoform lacks enzymatic activity, simple incubation with FMN activated the enzyme. It was stable enough to be crystallized, enabling us to determine its structure by X‐ray crystallography at 1.6 Å resolution. We also determined the FMN‐bound form at 1.8 Å resolution. Although the two structures have essentially the same scaffold, we observed flipping of a peptide‐bond plane in the vicinity of the FMN‐binding site, accompanied by an alternative hydrogen‐bonding pattern. Comparisons of B factors of the protein main chain revealed that binding of FMN decreased flexibility of most of the residues at the FMN‐binding site, but increased flexibility of a lid‐like loop structure over the active center. This increase was ascribed to a conformational change in an FMN‐contacting residue, Asn195, which induced a rearrangement of a hydrogen‐bond network of the residues comprising the lid. Abstract : We determined crystal structures of dihydroorotate dehydrogenase, a flavin mononucleotide (FMN)‐binding enzyme, from Trypanosoma brucei in both the FMN‐bound and FMN‐free forms. Although they have the same scaffold, we observed flipping of a peptide‐bond plane near the FMN‐binding site, accompanied by an alternative hydrogen‐bonding pattern. Comparisons of B factors revealed that binding of FMN increased flexibility of the lid‐like loop.