An efficient and non-destructive DNA extraction method for oribatid mites. (25th February 2018)

• Record Type: Journal Article
• Authors: Ahaniazad, Mansoureh; Bagheri, Mohammad; Roumi, Vahid; Akrami, Mohammad Ali
• Is Part Of: Archives of phytopathology and plant protection. Volume 51:Number 3/4(2018)
• Page Start: 187
• Page End: 196
• Publication Date: 2018-02-25
• Subjects: CTAB -- TNES buffer -- DNA -- Acari, oribatida; Plants, Protection of -- Periodicals; Plant diseases -- Periodicals; 632
• DOI: 10.1080/03235408.2018.1444003
• Languages: English
• ISSNs: 0323-5408

Abstract

Abstract: Molecular methods play an important role in systematic acarology and DNA extraction is the first step in this ground. Nowadays, the varieties of methods are being used for extraction of DNA, but most of them destroy the important external characters that are essential for morphological identification. In order to overcome the problem of associating molecular and morphological information, we have developed a simple, efficient and non-destructive DNA extraction method for oribatid mites. The non-destructive method was tested on three different species [ Oppia nitens C.L. Koch, 1836 (Oppiidae), Acrotritia ardua C.L. Koch, 1841 (Euphthiracaridae), and Amerus polonicus Kulczynski, 1902 (Ameridae)] and exoskeleton of specimens were preserved in Hoyer's medium on glass microscopic slides for further identification via morphological studies. This method is more time consuming than commercial kits, but is easier and cheaper, meanwhile, allows systematic analysis with linking the morphological and genetic traits from a single mite. The most important advantage of TNES method is that after using this non-destructive method, specimens preserve all of their morphological features.