Depth-specific distribution and importance of nitrite-dependent anaerobic ammonium and methane-oxidising bacteria in an urban wetland. (April 2015)

• Record Type: Journal Article
• Authors: Shen, Li-dong; Liu, Shuai; He, Zhan-fei; Lian, Xu; Huang, Qian; He, Yun-feng; Lou, Li-ping; Xu, Xiang-yang; Zheng, Ping; Hu, Bao-lan
• Is Part Of: Soil biology and biochemistry. Volume 83(2015)
• Page Start: 43
• Page End: 51
• Publication Date: 2015-04
• Subjects: Anammox -- n-damo -- Vertical distribution -- Niche segregation -- Importance -- Wetlands; Soil biochemistry -- Periodicals; Soil biology -- Periodicals; Sols -- Biochimie -- Périodiques; Sols -- Biologie -- Périodiques; Sols -- Microbiologie -- Périodiques; Bodembiologie; Biochemie; 631.46
• DOI: 10.1016/j.soilbio.2015.01.010
• Languages: English
• ISSNs: 0038-0717

Abstract

Abstract: Anaerobic ammonium oxidation (anammox) and nitrite-dependent anaerobic methane oxidation (n-damo) are two recently discovered processes in the nitrogen cycle that are catalysed by anammox bacteria and n-damo bacteria, respectively. Here, the depth-specific distribution and importance of anammox bacteria and n-damo bacteria were studied in an urban wetland, Xixi Wetland, Zhejiang Province (China). Anammox bacteria related to Candidatus Brocadia, Candidatus Kuenenia and Candidatus Anammoxoglobus, and n-damo bacteria related to " Candidatus Methylomirabilis oxyfera" were present in the collected soil samples. The abundance of anammox bacteria (2.6–8.6 × 10 6 copies g −1 dry soil) in the shallow soils (0–10 cm and 20–30 cm) was higher than that (2.5–9.8 × 10 5 copies g −1 dry soil) in the deep soils, whereas the abundance of n-damo bacteria (0.6–1.3 × 10 7 copies g −1 dry soil) in the deep soils (50–60 cm and 90–100 cm) was higher than that (3.4–4.5 × 10 6 copies g −1 dry soil) in the shallow soils. Anammox activity was detected at all depths, and higher potential rates (12.1–21.4 nmol N2 g −1 dry soil d −1 ) were observed at depths of 0–10 cm and 20–30 cm compared with the rates (3.5–8.7 nmol N2 g −1 dry soil d −1 ) measured at depths of 50–60 and 90–100 cm. In contrast, n-damo was mainly occurred at depths of 50–60 cm and 90–100 cm with potential rates of 0.7–5.0 nmol CO2 g −1 dry soil d −1 . This study suggested the niche segregation of the anammox bacteria and n-damo bacteria in wetland soils, with anammox bacteria being active primarily in deep soils and n-damo bacteria being active primarily in shallow soils. Highlights: The co-occurrence of anammox and n-damo processes was confirmed in wetland soils. The surface wetland soils are the preferred habitats for the anammox process. The deep wetland soils are the preferred habitats for the n-damo process. The two processes play important roles in the nitrogen and methane cycles of wetland.