SNP g.1007A>G within the porcine DNAL4 gene affects sperm motility traits and percentage of midpiece abnormalities. (12th December 2017)

• Record Type: Journal Article
• Authors: Wiedemann, I; Maehlmeyer, A; Jansen, S; Sharifi, AR; Knorr, C
• Is Part Of: Reproduction in domestic animals. Volume 53:Number 2(2018)
• Page Start: 401
• Page End: 413
• Publication Date: 2017-12-12
• Subjects: Animal breeding -- Periodicals; Veterinary pathology -- Periodicals; Livestock -- Breeding -- Periodicals; 636.082
• DOI: 10.1111/rda.13120
• Languages: English
• ISSNs: 0936-6768

Abstract

Contents: The flagellar beating of a spermatozoa's axoneme is caused by the varying activation and inactivation of dynein molecules. Dynein, axonemal, light chain 4 ( DNAL4 ) is a functional candidate gene for sperm motility as it encodes a small subunit of the dyneins. We resequenced the porcine DNAL4 using three artificial insemination (AI) boars each with high (>68%) or low (<60%) motility, and detected 23 SNP. These were then genotyped for 82 AI boars. Using spermatological records, significantly negative genetic correlations between ejaculate volume (VOL) and the further spermatological parameters concentration (CONC) ( r = −.43), motility of undiluted semen (MOTUD) ( r = −.09), motility after 24 h (MOT1) ( r = −.17) and after 48 hr (MOT2) ( r = −.23) were estimated. Significantly positive correlations existed between CONC and MOT1 ( r = .07) as well as MOT2 ( r = .10), between MOTUD and MOT1 ( r = .33), between MOTUD and MOT2 ( r = .36), and finally between MOT1 and MOT2 ( r = .70). Significantly negatively correlated were all motility traits with the parameters abnormal acrosome (AA) (MOTUD r = −.06; MOT1 r = −.08, and MOT2 r = −.1) and presence of cytoplasmic droplet (CD) (MOTUD r = −.07; MOT1 r = −.08; MOT2 r = −.07). Association analyses (single marker regression model; SMR) propose that SNP g.1007A>G, located in the second intron, reduces motility significantly (MOTUD ‐4.59%; MOT1 ‐10.33%; MOT2 ‐19.37%). According to the dominant‐recessive model (DRM), genotype AA is always superior compared to genotypes AG and GG (i.e. MOTUD 67.67%, 64.16% and 53.91%; MOT1 54.17%, 43.75% and 28.44%; MOT2 44.12%, 24.91% and 4.97%). The average effect of gene substitution (g.1007A>G) on abnormal midpiece (AM) was 0.71%, the genotypic values—as expressed by LSmeans—were 0.1 (AA) and 0.81 (AG).