Development and validation of LC-MS/MS method for the determination of Ochratoxin A and its metabolite Ochratoxin α in poultry tissues and eggs. Issue 5 (4th May 2018)

• Record Type: Journal Article
• Authors: Paoloni, Angela; Solfrizzo, Michele; Bibi, Rita; Pecorelli, Ivan
• Is Part Of: Journal of environmental science and health. Volume 53:Issue 5(2018)
• Page Start: 327
• Page End: 333
• Publication Date: 2018-05-04
• Subjects: Mass-spectrometry -- poultry tissues -- eggs -- method validation -- OTA -- OTα; Pesticides -- Environmental aspects -- Periodicals; Food contamination -- Periodicals; Agricultural wastes -- Environmental aspects -- Periodicals; Ecology -- periodicals; Pesticides -- toxicity -- periodicals; Food Contamination -- periodicals; 363.73
• DOI: 10.1080/03601234.2018.1431455
• Languages: English
• ISSNs: 0360-1234

Abstract

ABSTRACT: The objective of this study was to develop a liquid chromatography tandem mass spectrometry (LC-MS/MS) method for the determination of Ochratoxin A (OTA) and Ochratoxin α (OTα) in poultry tissues and eggs. The two toxins were extracted by a mixture of acetonitrile/water, purified with a reversed phase C18 solid phase extraction column (SPE) and determined by LC-MS/MS. The LC-MS/MS method performances were evaluated in terms of linearity in solvent and in matrix (ranged from 0.5 to 15.10 µg L −1 for OTA and from 0.60 to 17.85 µg L −1 for OTα), limit of detection (LOD), limit of quantitation (LOQ), specificity, accuracy and precision in repeatability conditions. Recovery experiments were performed by spiking poultry liver, kidney, muscle and eggs around 1 µg kg −1 and 10 µg kg −1 . LODs were 0.27 and 0.26 µg kg −1 while LOQs were fixed at 1.0 and 1.2 µg kg −1 for OTA and OTα, respectively. Main recoveries for OTA ranged from 82 to 109% and for OTα ranged from 55 to 89%. The values of within-laboratory relative standard deviation (RSDr ) were equal to or below 20%. Considering the results obtained and that all analytical performance criteria were fulfilled, the new extraction and purification method developed for OTA and OTα determination in animal tissues and eggs was found appropriate for control laboratories and research activities designed to ensure food safety.