Isothermal multiple displacement amplification of DNA templates in minimally buffered conditions using phi29 polymerase. Issue 17 (12th February 2018)
- Record Type:
- Journal Article
- Title:
- Isothermal multiple displacement amplification of DNA templates in minimally buffered conditions using phi29 polymerase. Issue 17 (12th February 2018)
- Main Title:
- Isothermal multiple displacement amplification of DNA templates in minimally buffered conditions using phi29 polymerase
- Authors:
- Tenaglia, Enrico
Imaizumi, Yuki
Miyahara, Yuji
Guiducci, Carlotta - Abstract:
- Abstract : phi29 can be used to amplify DNA and at constant temperature and minimally buffered conditions to produce pH readouts. Abstract : The isothermal amplification of DNA in minimally buffered conditions allows to perform and monitor nucleic acid amplification with minimal technological and operative requirements. We show in this work how phi29 can operate multiple displacement amplification in minimally buffered conditions producing, as a readout, pH shifts attaining subunits of pH.
- Is Part Of:
- Chemical communications. Volume 54:Issue 17(2018)
- Journal:
- Chemical communications
- Issue:
- Volume 54:Issue 17(2018)
- Issue Display:
- Volume 54, Issue 17 (2018)
- Year:
- 2018
- Volume:
- 54
- Issue:
- 17
- Issue Sort Value:
- 2018-0054-0017-0000
- Page Start:
- 2158
- Page End:
- 2161
- Publication Date:
- 2018-02-12
- Subjects:
- Chemistry -- Periodicals
540 - Journal URLs:
- http://pubs.rsc.org/en/journals/journalissues/cc ↗
http://www.rsc.org/ ↗ - DOI:
- 10.1039/c7cc09609g ↗
- Languages:
- English
- ISSNs:
- 1359-7345
- Deposit Type:
- Legaldeposit
- View Content:
- Available online (eLD content is only available in our Reading Rooms) ↗
- Physical Locations:
- British Library DSC - 3139.350000
British Library DSC - BLDSS-3PM
British Library STI - ELD Digital store - Ingest File:
- 6150.xml