Identification of isoforms of aspartic acid residues in peptides by 2D UV-MS fingerprinting of cold ions. Issue 4 (30th January 2018)

• Record Type: Journal Article
• Authors: Kopysov, Vladimir; Gorshkov, Mikhail V.; Boyarkin, Oleg V.
• Is Part Of: Analyst. Volume 143:Issue 4(2018)
• Page Start: 833
• Page End: 836
• Publication Date: 2018-01-30
• Subjects: Chemistry, Analytic -- Periodicals; 543
• DOI: 10.1039/c7an02044a
• Languages: English
• ISSNs: 0003-2654

Abstract

Abstract : We use 2D UV-MS cold-ion spectroscopy for the identification ofl -Asp, d -Asp, l -isoAsp andd -isoAsp residues in a fragment peptide derived from the hormone protein amylin. Abstract : We use 2D UV-MS cold-ion spectroscopy for the identification ofl -Asp, d -Asp, l -isoAsp andd -isoAsp residues in a fragment peptide derived from the hormone protein amylin. Relative solution concentrations of all four isoforms in an equimolar quaternary mixture have been determined within 4% error. This method demonstrates that for binary mixtures of the peptides an accuracy of 2.5% can be reached in few-second measurements.