Molecular mechanisms of dust-induced toxicity in human corneal epithelial cells: Water and organic extract of office and house dust. (July 2016)

• Record Type: Journal Article
• Authors: Xiang, Ping; Liu, Rong-Yan; Sun, Hong-Jie; Han, Yong-He; He, Rui-Wen; Cui, Xin-Yi; Ma, Lena Q.
• Is Part Of: Environment international. Volume 92/93(2016:Jul.)
• Page Start: 348
• Page End: 356
• Publication Date: 2016-07
• Subjects: Indoor dust -- Primary human corneal epithelial cells -- Oxidative stress -- Pro-inflammation -- NF-κB activation -- Polycyclic aromatic hydrocarbons -- Heavy metals; Environmental protection -- Periodicals; Environmental health -- Periodicals; Environmental monitoring -- Periodicals; Environmental Monitoring -- Periodicals; Environnement -- Protection -- Périodiques; Hygiène du milieu -- Périodiques; Environnement -- Surveillance -- Périodiques; Environmental health; Environmental monitoring; Environmental protection; Periodicals; 333.705
• DOI: 10.1016/j.envint.2016.04.013
• Languages: English
• ISSNs: 0160-4120

Abstract

Abstract: Human corneal epithelial (HCE) cells are continually exposed to dust in the air, which may cause corneal epithelium damage. Both water and organic soluble contaminants in dust may contribute to cytotoxicity in HCE cells, however, the associated toxicity mechanisms are not fully elucidated. In this study, indoor dust from residential houses and commercial offices in Nanjing, China was collected and the effects of organic and water soluble fraction of dust on primary HCE cells were examined. The concentrations of heavy metals in the dust and dust extracts were determined by ICP-MS and PAHs by GC–MS, with office dust having greater concentrations of heavy metals and PAHs than house dust. Based on LC50, organic extract was more toxic than water extract, and office dust was more toxic than house dust. Accordingly, the organic extracts induced more ROS, malondialdehyde, and 8-Hydroxydeoxyguanosine and higher expression of inflammatory mediators ( IL-1β, IL-6, and IL-8 ), and AhR inducible genes ( CYP1A1, and CYP1B1 ) than water extracts ( p < 0.05). Extracts of office dust presented greater suppression of superoxide dismutase and catalase activity than those of house dust. In addition, exposure to dust extracts activated NF-κB signal pathway except water extract of house dust. The results suggested that both water and organic soluble fractions of dust caused cytotoxicity, oxidative damage, inflammatory response, and activation of AhR inducible genes, with organic extracts having higher potential to induce adverse effects on primary HCE cells. The results based on primary HCE cells demonstrated the importance of reducing contaminants in indoor dust to reduce their adverse impacts on human eyes. Graphical abstract: Highlights: First report on molecular toxicity of water & organic extracts (WE & OE) of dust on primary human corneal epithelial cells OE elicited more ROS, MAD, and 8-OHdG and higher expression of inflammatory mediators, and AhR inducible genes than WE Dust extracts activated NF-κB signal pathway except water extract of house dust OE were more potent than WE, and office dust was more potent than house dust to induce adverse effects in cells