Biopolymeric nanogel derived from functionalized glycogen towards targeted delivery of 5-fluorouracil. (28th March 2018)

• Record Type: Journal Article
• Authors: Patra, Priyapratim; Seesala, Venkata Sundeep; Das, Dipankar; Panda, Asit Baran; Dhara, Santanu; Pal, Sagar
• Is Part Of: Polymer. Volume 140(2018)
• Page Start: 122
• Page End: 130
• Publication Date: 2018-03-28
• Subjects: Glycogen -- Nanogel -- 5-Fluorouracil; Polymers -- Periodicals; Polymerization -- Periodicals; Polymères -- Périodiques; Polymérisation -- Périodiques; 547.7
• DOI: 10.1016/j.polymer.2018.02.015
• Languages: English
• ISSNs: 0032-3861

Abstract

Abstract: We report the synthesis, characterization and application of a novel biocompatible nanogel (n-Gly/pHEMA) synthesized from glycogen and 2-hydroxyethyl methacrylate using ethylene glycol dimethacrylate crosslinker. Here mesoporous silica has been used as template. The structure of the nanogel has been investigated with the help of FTIR, 1 H NMR and 13 C NMR spectroscopy. The FESEM, HR-TEM and DLS analyses exhibit that the size of the nanogel is in the range of 10–50 nm. Thermal degradation kinetics suggests the higher stability of the n-Gly/pHEMA nanogel after crosslinking. The gel strength has been assessed by measurement of rheological parameters. The native nanogel shows a good compatibility with MG-63 cell lines. The n-Gly/pHEMA nanogel can effectively load 5-fluorouracil (5-FU) with encapsulation efficiency of ∼60% after 48 h. The intercellular release experiment demonstrates that 5-FU loaded nanogel can efficiently kill the MG-63 cancer cells. In vitro release profile demonstrates controlled release behaviour with higher 5-FU release rate at pH 5.5 compared to pH 7.4. Graphical abstract: Highlights: Crosslinked glycogen based nanogel has been synthesized as 5-fluorouracil carrier. The developed nanogel is non-cytotoxic. 5-Flourouracil loaded nanogel efficiently kills MG-63 osteosarcoma cancer cells.