Development of a novel dental resin cement incorporating FGF-2-loaded polymer particles with the ability to promote tissue regeneration. Issue 4 (April 2018) - Tsuboi, Ririko
Highlights: FGF-2-loaded polyHEMA/TMPT particles were prepared and freeze dried. FGF-2-loaded particles could be incorporated in a 4-META/MMA-based adhesive resin. FGF-2 release from particles incorporated in the resin was sustained for 14 days. FGF-2 released from particles incorporated in the resin maintained its bioactivity. In vivo results indicated that the experimental resin promoted bone regeneration. Abstract: Objective: Aiming to achieve bioactive dental resins that promote healing of surrounding tissues, we developed novel poly(2-hydroxyethyl methacrylate/trimethylolpropane trimethacrylate) (polyHEMA/TMPT) particles. These particles have been reported to be useful as a non-biodegradable carrier for fibroblast growth factor-2 (FGF-2) in vitro . The aim of this study was to evaluate the ability of an adhesive resin incorporating FGF-2-loaded polymer particles to promote tissue regeneration in vitro and in vivo . Methods: Experimental adhesive resins were prepared by incorporating FGF-2-loaded polyHEMA/TMPT particles into a 4-META/MMA-based adhesive resin, and the release profiles of FGF-2 were evaluated. The proliferation of osteoblast-like cells in the eluate from cured experimental resin was assessed. When the experimental resin was implanted into rat calvaria defects, bone regeneration was evaluated by microcomputed tomography and histological observations. Results: Sustained release of FGF-2 from the experimental resin was observed for 14 days. Eluate from the cured experimental resin significantly promoted the proliferation of osteoblast-like cells. Significantly greater bone regeneration was observed using the experimental resin compared with the control resin without FGF-2. Significance: 4-META/MMA-based adhesive resin incorporating FGF-2-loaded polymer particles is useful to promote tissue regeneration, suggesting that its application would be beneficial for root-end filling or the repair of fractured roots in cases with severely damaged periodontal tissue.