Structure elucidation of the capsular polysaccharide of Acinetobacter baumannii AB5075 having the KL25 capsule biosynthesis locus. (18th May 2015)

• Record Type: Journal Article
• Authors: Senchenkova, Sof'ya N.; Shashkov, Alexander S.; Popova, Anastasiya V.; Shneider, Mikhail M.; Arbatsky, Nikolay P.; Miroshnikov, Konstantin A.; Volozhantsev, Nikolay V.; Knirel, Yuriy A.
• Is Part Of: Carbohydrate research. Volume 408(2015)
• Page Start: 8
• Page End: 11
• Publication Date: 2015-05-18
• Subjects: Acinetobacter baumannii -- Capsular polysaccharide structure -- Polysaccharide gene locus; Carbohydrates -- Periodicals; Chemistry, Organic -- Periodicals; Biochemistry -- Periodicals; Carbohydrates -- Periodicals; Chimie organique -- Périodiques; Glucides -- Périodiques; Biochemistry; Carbohydrates; Chemistry, Organic; Periodicals; Electronic journals; 507.78
• DOI: 10.1016/j.carres.2015.02.011
• Languages: English
• ISSNs: 0008-6215

Abstract

Abstract: Capsular polysaccharide was isolated by the phenol–water extraction of Acinetobacter baumannii AB5075 and studied by 1D and 2D 1 H and 13 C NMR spectroscopy. The following structure of the linear trisaccharide repeating unit was established: →3)-β-d -Man p NAcA-(1→4)-β-d -Man p NAcA-(1→3)-α-d -Qui p NAc4NR-(1→ where R indicates ( S )-3-hydroxybutanoyl or acetyl in the ratio∼2.5:1. The genes in the polysaccharide biosynthesis locus designated KL25 are appropriate to the established CPS structure. Graphical abstract: Highlights: Capsular polysaccharide was isolated from Acinetobacter baumannii AB5075 Structure of the polysaccharide was established by NMR and chemical approaches Capsule biosynthesis locus was analyzed and found to match the structure established